Differential Effect of Viable Versus Necrotic Neutrophils on Mycobacterium tuberculosis Growth and Cytokine Induction in Whole Blood

Frontiers in Immunology
David M LoweAdrian R Martineau

Abstract

Neutrophils exert both positive and negative influences on the host response to tuberculosis, but the mechanisms by which these differential effects are mediated are unknown. We studied the impact of live and dead neutrophils on the control of Mycobacterium tuberculosis using a whole blood bioluminescence-based assay, and assayed supernatant cytokine concentrations using Luminex™ technology and ELISA. CD15+ granulocyte depletion from blood prior to infection with M. tuberculosis-lux impaired control of mycobacteria by 96 h, with a greater effect than depletion of CD4+, CD8+, or CD14+ cells (p < 0.001). Augmentation of blood with viable granulocytes significantly improved control of mycobacteria by 96 h (p = 0.001), but augmentation with necrotic granulocytes had the opposite effect (p = 0.01). Both augmentations decreased supernatant concentrations of tumor necrosis factor and interleukin (IL)-12 p40/p70, but necrotic granulocyte augmentation also increased concentrations of IL-10, G-CSF, GM-CSF, and CCL2. Necrotic neutrophil augmentation reduced phagocytosis of FITC-labeled M. bovis BCG by all phagocytes, whereas viable neutrophil augmentation specifically reduced early uptake by CD14+ cells. The immunosuppressive effect of de...Continue Reading

References

Dec 15, 1996·The Journal of Clinical Investigation·D L PitrakP Stevens
Aug 24, 1999·Infection and Immunity·V A SnewinD B Young
Aug 19, 2000·The Journal of Infectious Diseases·B KampmannM Levin
Oct 27, 2004·Infection and Immunity·Beate KampmannMichael Levin
Feb 26, 2005·Infection and Immunity·Evgenyi B EruslanovAlexander S Apt
Jul 4, 2007·The Journal of Clinical Investigation·Adrian R MartineauRobert J Wilkinson
Dec 22, 2007·Pathology, Research and Practice·Almério L L de NoronhaManoel Barral-Netto
Jul 15, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katherine MilesMohini Gray
Jul 26, 2011·Nature Reviews. Immunology·Alberto MantovaniSébastien Jaillon
Sep 9, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C-Y ChenP-R Hsueh
Nov 19, 2011·Trends in Immunology·David M LoweAdrian R Martineau
Jan 22, 2013·Tuberculosis·David M LoweAdrian R Martineau
Oct 12, 2013·The European Respiratory Journal·David M LoweAdrian R Martineau
May 4, 2015·Tuberculosis·Vladimir YeremeevAlexander Apt
Sep 15, 2015·Annals of the American Thoracic Society·David M LoweAdrian R Martineau
Dec 10, 2015·Nature·Jacqueline M KimmeyChristina L Stallings
Jul 28, 2016·The Journal of Infectious Diseases·Collin R DiedrichRobert J Wilkinson

❮ Previous
Next ❯

Citations

Nov 14, 2019·Cellular and Molecular Life Sciences : CMLS·Qiyao ChaiCui Hua Liu
Apr 3, 2020·Clinical Microbiology Reviews·Jansy P Sarathy, Véronique Dartois
Nov 30, 2018·Frontiers in Immunology·Elouise E KroonErwin Schurr
Sep 12, 2019·Frontiers in Pediatrics·Maurizio de MartinoElena Chiappini
May 6, 2021·International Journal of Molecular Sciences·Rachana R BorkuteAnca Dorhoi
Jul 28, 2021·Tuberculosis·Bartolo TamburiniNadia Caccamo

❮ Previous
Next ❯

Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
bronchoalveolar lavage
PCA
ELISA

Software Mentioned

Luminex
FlowJo
Treestar
[UNK] Omics Explorer
GraphPad Prism
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.