Differential effects of α-catenin on the invasion and radiochemosensitivity of human colorectal cancer cells

International Journal of Oncology
Sarah FörsterNils Cordes

Abstract

Driven by genetic and epigenetic alterations, progression, therapy resistance and metastasis are frequent events in colorectal cancer (CRC). Although often speculated, the function of cell-cell contact for radiochemosensitivity, particularly associated with E-cadherin/catenin complex, warrants further clarification. In this study, we investigated the role of the E-cadherin/catenin complex proteins under more physiological three-dimensional (3D) cell culture conditions in a panel of CRC cell lines. In contrast to floating spheroids and growth in the laminin-rich matrix, collagen type 1 induced the formation of two distinct growth phenotypes, i.e., cell groups and single cells, in 5 out of the 8 CRC cell lines. Further characterization of these subpopulations revealed that, intriguingly, cell-cell contact proteins are important for invasion, but negligible for radiochemosensitivity, proliferation and adhesion. Despite the generation of genomic and transcriptomic data, we were unable to elucidate the mechanisms through which α-catenin affects collagen type 1 invasion. In a retrospective analysis of patients with rectal carcinoma, a low α-catenin expression trended with overall survival, as well as locoregional and distant control....Continue Reading

References

Dec 1, 1987·Archives of Surgery·G W DanekerG D Steele
Dec 1, 1981·The American Journal of Medicine·D L DexterP Calabresi
Sep 19, 2000·International Journal of Radiation Biology·H MoussaM C Joiner
Nov 13, 2002·The Journal of Cell Biology·Renee C IretonAlbert B Reynolds
Dec 20, 2002·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·Viktor MeinekeDirk van Beuningen
Feb 26, 2003·Cell·Mirna Perez-MorenoElaine Fuchs
May 25, 2004·International Journal of Experimental Pathology·Mona El-BahrawyIan T Tomlinson
Oct 6, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Joerg SchlingemannMeinhard Hahn
Aug 15, 2006·Current Opinion in Cell Biology·René-Marc MègeMireille Lambert
Dec 30, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Claus RödelRolf Sauer
Dec 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kentaro Abe, Masatoshi Takeichi
Aug 30, 2008·Cellular and Molecular Life Sciences : CMLS·F van Roy, G Berx
Jun 3, 2009·The Journal of Clinical Investigation·Michael Zeisberg, Eric G Neilson
Jul 7, 2009·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·A I FilizM L Akin
Sep 12, 2009·The American Journal of Pathology·Claire L PlumbBrenda L Coomber
Jun 10, 2010·The Journal of Clinical Investigation·Iris EkeNils Cordes
Jun 28, 2011·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Iris Eke, Nils Cordes
Oct 12, 2011·Cell and Tissue Research·Vivien J Coulson-ThomasHelena B Nader
Mar 26, 2013·Journal of Cell Science·Marta CanelValerie G Brunton
Sep 21, 2013·Nature·Rebecca A BurrellCharles Swanton
Feb 28, 2014·World Journal of Gastroenterology : WJG·Alexander Stein, Carsten Bokemeyer
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Rebecca L DaughertyCara J Gottardi
Oct 19, 2014·Journal of Cancer Research and Clinical Oncology·Dandan YuanFeng Bi
Jan 24, 2015·Gastroenterology Research and Practice·Charalampos C Mylonas, Andreas C Lazaris
Jun 23, 2015·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Mandy PoschauNils Cordes
Jul 3, 2015·Cell Communication & Adhesion·Otmar Huber, Iver Petersen
Jan 1, 2015·Nature Reviews. Disease Primers·Ernst J KuipersToshiaki Watanabe
Jun 8, 2017·Oncology Letters·Niki ChristouMuriel Mathonnet

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE109047

Methods Mentioned

BETA
transfection
biopsy
biopsies
X-ray

Software Mentioned

GenePattern
Excel
Microarray Suite
cytoscape
Affymetrix Gene Expression Console
ImageJ
SPSS
Prism
GraphPad
Affymetrix Chromosome Analysis Suite ( ChAS )

Related Concepts

Related Feeds

Cell Signaling & Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. This feed covers the latest research on signaling and epigenetics in cell growth and cancer.

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.

Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cancer Epigenetics

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

Cancer Epigenetics & Methyl-CpG (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. Here is the latest research on cancer epigenetics and methyl-CpG binding proteins including ZBTB38.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.