Differential effects of a GTP-restricted mutant of Sar1p on segregation of cargo during export from the endoplasmic reticulum

Journal of Cell Science
David J Stephens, Rainer Pepperkok

Abstract

Export of cargo from the endoplasmic reticulum (ER) is the first membrane trafficking step in the secretory pathway. To date, all cargo proteins appear to use a common set of machinery for the initial stages of export, namely the COPII coat complex. Recent data from both yeast and mammalian systems have emerged suggesting that specific cargoes could be sorted from one another at the point of exit from the endoplasmic reticulum or immediately afterwards. Here, we have examined the mechanisms used for export of different types of cargo molecule from the endoplasmic reticulum. All cargoes examined utilise the COPII machinery, but specific differences are seen in the accumulation of cargo into ER-derived pre-budding complexes following expression of a GTP-restricted mutant of the Sar1p GTPase. Glycosylphosphatidylinositol (GPI)-anchored GFP is seen to be restricted to the ER under these conditions whereas other cargoes, including ts045-G and lumFP accumulate in pre-budding complexes. Following exit, GPI-FP, lumFP and ts045-G-FP all travel to the Golgi in the same vesicular tubular clusters (VTCs). These data show a differential requirement for efficient GTP hydrolysis by the Sar1p GTPase in export of cargo from the ER.

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Citations

Dec 7, 2007·Histochemistry and Cell Biology·Helen Hughes, David J Stephens
Sep 5, 2008·Molecular Biology of the Cell·Laura A SchroderWilliam A Dunn
Mar 5, 2010·Molecular Biology of the Cell·Polina Shindiapina, Charles Barlowe
Apr 10, 2009·The Journal of Pharmacology and Experimental Therapeutics·Xiaoping ZhangGuangyu Wu
Dec 15, 2010·Molecular Membrane Biology·Katy Schmidt, David J Stephens
Oct 22, 2011·Trends in Pharmacological Sciences·Guansong Wang, Guangyu Wu
Jun 11, 2011·Progress in Lipid Research·Yusuke Maeda, Taroh Kinoshita
Aug 19, 2007·Seminars in Cell & Developmental Biology·Semra J Kirk, Theresa H Ward
Sep 19, 2009·The EMBO Journal·Kazuhito V TabataHiroyuki Noji
Dec 5, 2008·Traffic·Guillaume A CastillonHoward Riezman
Jun 6, 2007·Traffic·Vytaute Starkuviene, Rainer Pepperkok
Jan 25, 2006·Current Biology : CB·Rebecca ForsterRainer Pepperkok
Jun 28, 2005·Biochimica Et Biophysica Acta·Peter Watson, David J Stephens
Feb 6, 2014·Nature Reviews. Molecular Cell Biology·Alexandre A Mironov
Apr 29, 2020·The Journal of Cell Biology·Safa Lucken-Ardjomande HäslerHarvey T McMahon
Apr 13, 2007·The Journal of Biological Chemistry·Takayuki IinumaKatsuko Tani
Nov 2, 2005·The Journal of Cell Biology·Burcu HasdemirRobert D Burgoyne
Dec 15, 2020·Bioorganic & Medicinal Chemistry·Sanjeevini Babu ReddiarRaymond S Norton

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