Differential effects of AAV.BDNF and AAV.Ntf3 in the deafened adult guinea pig ear

Scientific Reports
Cameron L BudenzY Raphael

Abstract

Cochlear hair cell loss results in secondary regression of peripheral auditory fibers (PAFs) and loss of spiral ganglion neurons (SGNs). The performance of cochlear implants (CI) in rehabilitating hearing depends on survival of SGNs. Here we compare the effects of adeno-associated virus vectors with neurotrophin gene inserts, AAV.BDNF and AAV.Ntf3, on guinea pig ears deafened systemically (kanamycin and furosemide) or locally (neomycin). AAV.BDNF or AAV.Ntf3 was delivered to the guinea pig cochlea one week following deafening and ears were assessed morphologically 3 months later. At that time, neurotrophins levels were not significantly elevated in the cochlear fluids, even though in vitro and shorter term in vivo experiments demonstrate robust elevation of neurotrophins with these viral vectors. Nevertheless, animals receiving these vectors exhibited considerable re-growth of PAFs in the basilar membrane area. In systemically deafened animals there was a negative correlation between the presence of differentiated supporting cells and PAFs, suggesting that supporting cells influence the outcome of neurotrophin over-expression aimed at enhancing the cochlear neural substrate. Counts of SGN in Rosenthal's canal indicate that BDNF...Continue Reading

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·U PirvolaM Saarma
Apr 1, 1988·Hearing Research·P A Leake, G T Hradek
Apr 1, 1997·Trends in Neurosciences·B FritzschI Fariñas
Jul 27, 2002·The Journal of the Acoustical Society of America·Li XuBryan E Pfingst
Apr 6, 2004·Ear and Hearing·Ying-Yee KongFan-Gang Zeng
Jun 14, 2005·Journal of the Association for Research in Otolaryngology : JARO·Mitsuru SugawaraM Charles Liberman
May 24, 2006·Journal of the Association for Research in Otolaryngology : JARO·Robert MynattAlec N Salt
Jul 11, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Krystof S BankiewiczJanet Cunningham
Jan 9, 2007·The Journal of Comparative Neurology·Mitsuru SugawaraGabriel Corfas
Nov 20, 2009·Audiology & Neuro-otology·Alec N Salt, Stefan K Plontke
Jan 30, 2010·Experimental Neurology·Seiji B ShibataYehoash Raphael
May 19, 2011·Methods in Molecular Biology·James N WarnockMohamed Al-Rubeai
Oct 10, 2012·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Cameron L BudenzYehoash Raphael

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Citations

Aug 16, 2016·Molecular Therapy. Methods & Clinical Development·Min Young LeeYehoash Raphael
Aug 5, 2017·Journal of the Association for Research in Otolaryngology : JARO·Bryan E PfingstYehoash Raphael
Aug 30, 2018·Physiological Reviews·Jing Wang, Jean-Luc Puel
Dec 15, 2015·Journal of Neural Engineering·Stefan HahnewaldPascal Senn
Jan 30, 2020·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Thomas R Van De Water
Aug 21, 2019·Nature Communications·Fangzhi TanGuisheng Zhong
Oct 17, 2018·Cold Spring Harbor Perspectives in Medicine·Lawrence Lustig, Omar Akil
Apr 12, 2019·Frontiers in Neuroscience·Ines de VriesAthanasia Warnecke
Aug 6, 2019·Frontiers in Cellular Neuroscience·Yin RenKonstantina M Stankovic
Jun 7, 2020·Journal of Otolaryngology - Head & Neck Surgery = Le Journal D'oto-rhino-laryngologie Et De Chirurgie Cervico-faciale·Brian W BlakleyAbdulrahman Alenezi
Sep 10, 2020·Physiological Research·L HeS-S Gong
Jul 19, 2019·Hearing Research·Anne G M SchilderHelen Blackshaw
May 11, 2020·Advanced Drug Delivery Reviews·Ning DingXue Gao
Jan 1, 2021·The Journal of the Acoustical Society of America·Kara C Schvartz-LeyzacBryan E Pfingst
Apr 26, 2020·Hearing Research·Patricia A LeakeHainan Lang
Apr 15, 2020·Acta Biomaterialia·Fei TanMohamed Al-Rubeai
Jul 19, 2018·Molecular Therapy : the Journal of the American Society of Gene Therapy·Maria J DuarteDaniel J Lee

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Methods Mentioned

BETA
ELISA
dissection
light microscopy

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