Differential effects of acute hypoxia and endotoxin on the secretion and expression of bone marrow interleukin-1 and interleukin-6

Shock
A M MohrD H Livingston

Abstract

Hemorrhagic shock induces tissue hypoxia and has been demonstrated to alter the myelopoietic response to bacterial lipopolysaccharide (LPS). Interleukin-1 and interleukin-6 are important mediators of immunologic events after hemorrhagic shock. Bone marrow stroma release inflammatory cytokines, which may play a role in the regulation of myelopoiesis after injury. The aim of this study was to correlate cytokine gene expression with protein release and myelopoiesis by total bone marrow cells. The role of bone marrow stroma after exposure to hypoxia and lipopolysaccharide was also examined. BALB/c mice were designated as normoxia or hypoxia and total bone marrow cells were harvested. Hypoxia mice were exposed to 2 h of 5% O2/95% N2, and then returned to room air. Additional groups of mice were given LPS intraperitoneally. Bone marrow stroma, from BALB/c mice, was similarly designated. Myelopoiesis was assessed by growth of granulocyte-macrophage progenitor cells (CFU-GM). Interleukin-1 and interleukin-6 protein activity was assessed by bioassay. RNA was extracted from both total bone marrow cells and bone marrow stroma. By day 5, LPS alone resulted in a 93% increase in CFU-GM versus normoxia. Hypoxia and LPS exposure significantly ...Continue Reading

Citations

Jul 28, 2011·Surgical Infections·Ihab O ElhassanAlicia M Mohr
Sep 8, 2001·Critical Care Medicine·D J AnjariaD H Livingston
Aug 12, 2009·The Journal of Trauma·Salil ShahDavid H Livingston
Aug 12, 2010·The Journal of Trauma·Keith A BeiermeisterAlicia M Mohr
Dec 7, 2007·Brain, Behavior, and Immunity·Raghav G MurthyPranela Rameshwar
Jul 8, 2010·The Journal of Surgical Research·Angela PennDavid H Livingston
May 18, 2000·American Journal of Hematology·R TanejaD H Livingston
May 31, 2001·European Journal of Haematology·Z Ben-Ishay, V Barak
Oct 17, 2017·Current Opinion in Hematology·Tyler J LoftusLyle L Moldawer
Dec 31, 2002·The Journal of Surgical Research·Xiaosong SongDavid H Livingston

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