Differential effects of aromatic and charged residue substitutions in the RNA binding domains of the yeast poly(A)-binding protein

Journal of Molecular Biology
J A Deardorff, A B Sachs

Abstract

The yeast poly(A)-binding protein (Pab1p) contains four RNA recognition motifs (RRMs). Site-directed mutations were introduced into each of these RRMs in order to investigate their relative contributions to specific and non-specific RNA binding, and to determine the consequences of these mutations on the ability of Pab1p to support viability. Specifically, a charged and an aromatic residue that were predicted to be involved in RNA binding were mutated in each RRM. These mutations revealed that the second RRM is primarily responsible for poly(A) binding, while the fourth RRM is primarily responsible for non-specific polypyrimidine RNA binding. The mutated aromatic residues in each RRM contributed to both modes of binding whereas the mutated charged residues contributed primarily to non-specific RNA binding. RNA binding in vivo correlated with the in vitro binding measurements. Furthermore, RNA binding, but not high-affinity poly(A) binding, correlated with the ability of Pab1p to sustain yeast cell viability. These data suggest that a single aromatic substitution in Pab1p can significantly reduce its RNA binding ability, that the capacity of Pab1p to bind poly(A) as well as other RNAs is mediated by distinct residues within diff...Continue Reading

References

Nov 1, 1992·Genes & Development·J E LowellA B Sachs
Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Jun 1, 1991·Trends in Biochemical Sciences·D J KenanJ D Keene
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D W HoffmanJ D Keene
Jan 1, 1991·Methods in Enzymology·T A KunkelJ McClary
Aug 1, 1985·Molecular and Cellular Biology·A B Sachs, R D Kornberg
Mar 1, 1983·The Journal of Cell Biology·B W Baer, R D Kornberg
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B W Baer, R D Kornberg
Dec 1, 1995·Genes & Development·S Z Tarun, A B Sachs
Jul 1, 1994·Molecular and Cellular Biology·M E SamuelsP Schedl
Mar 11, 1995·Nucleic Acids Research·Y ShamooK R Williams
Apr 1, 1994·Experimental Cell Research·M GörlachG Dreyfuss
Feb 2, 1996·The Journal of Biological Chemistry·L M DemberP Anderson
May 3, 1996·The Journal of Biological Chemistry·A Proweller, J S Butler
Oct 1, 1996·Molecular and Cellular Biology·C E BrownA B Sachs

❮ Previous
Next ❯

Citations

May 28, 2004·Nature Structural & Molecular Biology·Tobias von der HaarJohn E G McCarthy
Jan 14, 2004·European Journal of Biochemistry·Rosemary T SladicGregory J Goodall
Jul 15, 2006·Nucleosides, Nucleotides & Nucleic Acids·Junji KawakamiYoshiatsu Tanabe
Feb 9, 2008·Nucleosides, Nucleotides & Nucleic Acids·Junji KawakamiNaoki Sugimoto
Aug 16, 2002·Molecular Biology of the Cell·Anjan G ThakurtaRavi Dhar
Apr 22, 2011·Molecular Biology of the Cell·Catherine A KonopkaRichard G Gardner
Aug 23, 2000·Nucleic Acids Research·O P de Melo NetoN Standart
Sep 20, 2006·Nucleic Acids Research·Sigrid D AuweterFrédéric H-T Allain
Apr 19, 2007·Nucleic Acids Research·Takbum OhnClyde L Denis
Feb 29, 2008·Nucleic Acids Research·Nicolas ViphakoneLionel Minvielle-Sebastia
Feb 11, 2010·Nucleic Acids Research·Mateusz WydroZofia M Chrzanowska-Lightowlers
Jun 10, 2000·Molecular and Cellular Biology·S ParkI A Laird-Offringa
May 9, 2002·Molecular and Cellular Biology·Guylaine RoyNahum Sonenberg
Jan 20, 2004·Molecular and Cellular Biology·Guylaine RoyNahum Sonenberg
Feb 17, 2005·Molecular and Cellular Biology·Gavin S WilkieNicola K Gray
Jul 17, 2007·Proceedings of the National Academy of Sciences of the United States of America·Seth M KellyKeith M Berland
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S M CrowderT Alber
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·S Z TarunA B Sachs
May 25, 2013·Nucleic Acids Research·Michael J LawIte A Laird-Offringa
Sep 24, 2016·Quarterly Reviews of Biophysics·Jin ChenJoseph D Puglisi
May 26, 2004·Biochimica Et Biophysica Acta·Uwe Kühn, Elmar Wahle
Nov 16, 2005·Comptes rendus biologies·Federica PiccioniArturo C Verrotti
Jan 20, 2017·Reproduction, Fertility, and Development·Saffet Ozturk, Fatma Uysal
Jul 28, 2018·Journal of Biomolecular Structure & Dynamics·Margarita I ZarudnayaDmytro M Hovorun
Feb 8, 2008·The EMBO Journal·Ann-Bin ShyuAmbro van Hoof
Mar 15, 2003·The Journal of Biological Chemistry·Uwe KühnElmar Wahle

❮ Previous
Next ❯

Related Concepts

Related Feeds

Auditory Perception

Auditory perception is the ability to receive and interpret information attained by the ears. Here is the latest research on factors and underlying mechanisms that influence auditory perception.