Differential effects of ca(2+) and Mg (2+) on endonuclease activation in isolated promyelocytic HL-60 cell nuclei

Science in China. Series C, Life Sciences
M FangS Xue

Abstract

In autodigestion assays, endonucleaw activity in non-apoptotic HL-60 promydocytic leukemia cell nuclei cleaved the chromatin of he autologous cells to an oligonucleosomal length pattern. Both EGTA and EDTA inhibited the activation of endonuclease activity in isolated HL-60 cell nuclei. The inhibition by EDTA could be reversed by exogenous Ca(2+). but not by exogenous Mg(2+). In Ca(2+)/Mg(2+)-free nuclei digation buffer, addition of Ca(2-->) (1-10 mmol/L) induced endonuclease activity in the isolated nuclei, while addition of Mg(2+) had no effect. In the presence of Ca(2+)(0.1 mmol/L), endonuclease activity was enhanced by exogenous Mg(2+) (0.1-10mmol/L). These results suggest that the endonuclease responsible for internucleosomal DNA fragmentation in HL-60 cells during apoptosis is activated by Ca(2+) and further modulated by Mg(2+) in the presence of ca(2+).

References

Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
Oct 1, 1994·Cell Calcium·P NicoteraS Orrenius
Dec 1, 1994·International Archives of Allergy and Immunology·B ZhivotovskyS Orrenius
Oct 29, 1993·Biochemical and Biophysical Research Communications·A YoshidaT Nakamura

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Citations

May 12, 2004·The Journal of Eukaryotic Microbiology·Myung Gil ParkD Wayne Coats

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