Differential effects of Ca2+ channel beta1a and beta2a subunits on complex formation with alpha1S and on current expression in tsA201 cells.

The Journal of Biological Chemistry
B NeuhuberB E Flucher

Abstract

To study the interactions of the alpha1S subunit of the skeletal muscle L-type Ca2+ channel with the skeletal beta1a and the cardiac beta2a, these subunits were expressed alone or in combination in tsA201 cells. Immunofluorescence- and green fluorescent protein-labeling showed that, when expressed alone, beta1a was diffusely distributed throughout the cytoplasm, beta2a was localized in the plasma membrane, and alpha1S was concentrated in a tubular/reticular membrane system, presumably the endoplasmic reticulum (ER). Upon coexpression with alpha1S, beta1a became colocalized with alpha1S in the ER. Upon coexpression with beta2a, alpha1S redistributed to the plasma membrane, where it aggregated in large clusters. Coexpression of alpha1S with beta1a but not with beta2a increased the frequency at which cells expressed L-type currents. A point mutation (alpha1S-Y366S) or deletion (alpha1S-Delta351-380) in the beta interaction domain of alpha1S blocked both translocation of beta1a to the ER and beta2a-induced translocation of the alpha1S mutants to the plasma membrane. However, the point mutation did not interfere with beta1a-induced current stimulation. Thus, beta1a and beta2a are differentially distributed in tsA201 cells and upon c...Continue Reading

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Citations

May 21, 2009·European Biophysics Journal : EBJ·Yamuna KarunasekaraMarco G Casarotto
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·B U FitzkyF F Moebius
Dec 31, 2014·Proceedings of the National Academy of Sciences of the United States of America·Alexander PolsterKurt G Beam
Jul 13, 2004·The European Journal of Neuroscience·Takahiro YasudaGerald W Zamponi
Jan 24, 2017·Scientific Reports·Marta Campiglio, Bernhard E Flucher
Aug 3, 2018·Journal of Cellular Physiology·Marta CampiglioBernhard E Flucher
Sep 13, 2007·Journal of Cellular Physiology·Robert A Maue
Jul 27, 2006·Endocrine Reviews·Shao-Nian Yang, Per-Olof Berggren
Mar 1, 2013·Journal of Cell Science·Marta CampiglioBernhard E Flucher
Jun 11, 2002·Biochemical and Biophysical Research Communications·Vladimir SerikovArnold Schwartz

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