PMID: 6411997Aug 1, 1983Paper

Differential effects of chelating agents on cytosolic glucocorticoid receptor stability and nuclear binding in vitro

Journal of Steroid Biochemistry
J R Hubbard, M Kalimi

Abstract

The effect of several metal chelators (EDTA, EGTA, and 1,10 phenanthroline) on rat liver glucocorticoid receptor properties in vitro was investigated. At 4 degrees C 10 mM EDTA (unlike 10 mM EGTA and 10 mM 1,10 phenanthroline) had a significant stabilizing effect on unbound hepatic glucocorticoid receptors. At higher temperature (25 degrees C) 10 mM EGTA appeared to act as a chemical stabilizer of unbound receptors. 1,10 Phenanthroline had no stabilizing effect at either temperature. Scatchard analysis indicated that the alteration in receptor binding after incubation at 4 and 25 degrees C in the presence and absence of chelating agents was due to a change in the number of steroid binding sites rather than perturbation of receptor affinity. Unlike results obtained with unbound receptors, all three chelating agents appeared to enhance prebound glucocorticoid-receptor complex inactivation. Interestingly these chelating reagents also significantly altered glucocorticoid-receptor complex binding to isolated nuclei.

References

Aug 15, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·R De PirroR Lauro
Jul 25, 1977·Biochemical and Biophysical Research Communications·Y Sakaue, E B Thompson
Jan 1, 1980·Hoppe-Seyler's Zeitschrift für physiologische Chemie·S SobollH W Heldt
Jan 1, 1980·Journal of Steroid Biochemistry·P Arányi, A Náray

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