PMID: 8947311Nov 1, 1996Paper

Differential effects of chronic ethanol consumption on hepatic mitochondrial and cytoplasmic ribosomes

Alcoholism, Clinical and Experimental Research
A CahillC C Cunningham

Abstract

The effects of chronic ethanol consumption on the properties of mitochondrial and cytoplasmic ribosomes were investigated in rat liver. Sedimentation properties of purified mitochondrial (55S) and cytoplasmic (80S) ribosomes were determined by analyses on sucrose density gradients. Mitochondrial ribosomes from control animals moved further in the gradients than did those isolated from ethanol-fed rats, which suggests that ethanol ribosomes have a lower molecular weight. In addition, mitochondrial from ethanol-fed animals contained a lower percentage of ribosomes present as the intact monosome, suggesting that ethanol may have an effect on the stability of the functional mitochondrial ribosomes. This was confirmed by the presence of the larger 39S subunit in preparations from ethanol-fed animals. No such ethanol-related alterations were seen with cytoplasmic ribosomes. The protein composition of mitochondrial cytoplasmic ribosomes was investigated using two-dimensional gel electrophoresis, followed by two-dimensional densitometry. As indicated by differences in protein staining intensity, ethanol consumption seemed to alter the concentration of seven mitochondrial ribosomal proteins. In contrast, no such changes were observed in...Continue Reading

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Citations

Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A CahillJ B Hoek
Jan 29, 2002·Archives of Biochemistry and Biophysics·Vinood B Patel, Carol C Cunningham
Jan 5, 2002·Free Radical Biology & Medicine·Shannon M Bailey, Carol C Cunningham
May 5, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J G Pastorino, J B Hoek
Apr 9, 1999·Alcoholism, Clinical and Experimental Research·L FanP A Wilce
Feb 26, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Brian WeiserAlan Cahill
Nov 3, 2004·Journal of Hepatology·Marie-Astrid PiquetXavier M Leverve
Feb 28, 2003·Alcoholism, Clinical and Experimental Research·Vinood B PatelVictor R Preedy
Jun 18, 1997·Biochemical and Biophysical Research Communications·A CahillJ B Hoek
Jun 8, 2001·Alcoholism, Clinical and Experimental Research·C C CunninghamK Torii
Jul 11, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shannon M BaileyVictor Darley-Usmar
Nov 21, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Qian SunZhanxiang Zhou
Dec 7, 2000·The Journal of Biological Chemistry·V B PatelR R Hantgan
Mar 23, 2004·The Journal of Biological Chemistry·Aparna VenkatramanShannon M Bailey
Jun 26, 1999·Gastroenterology·A I Cederbaum

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