Differential effects of detergents on keratinocyte gene expression

The Journal of Investigative Dermatology
F Van RuissenJ Schalkwijk

Abstract

We have studied the effect of various detergents on keratinocyte gene expression in vitro, using an anionic detergent (sodium dodecyl sulfate), a cationic detergent cetyltrimethylammoniumbromide (CTAB), and two nonionic detergents, Nonidet P-40 and Tween-20. We measured the effect of these detergents on direct cellular toxicity (lactate dehydrogenase release), on the expression of markers for normal differentiation (cytokeratin 1 and involucrin expression), and on disturbed keratinocyte differentiation (SKALP) by northern blot analysis. As reported in other studies, large differences were noted in direct cellular toxicity. In a culture model that mimics normal epidermal differentiation we found that low concentrations of sodium dodecyl sulfate could induce the expression of SKALP, a proteinase inhibitor that is not normally expressed in human epidermis but is found in hyperproliferative skin. Sodium dodecyl sulfate caused upregulation of involucrin and downregulation of cytokeratin 1 expression, which is associated with the hyperproliferative/inflammatory epidermal phenotype found in psoriasis, wound healing, and skin irritation. These changes were not induced after treatment of cultures with CTAB, Triton X-100, and Nonidet-P40...Continue Reading

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Citations

Mar 26, 2002·The Pharmacogenomics Journal·J L OestreicherW L Trepicchio
Dec 10, 1999·The Journal of Clinical Investigation·W L TrepicchioJ G Krueger
Jul 24, 2012·BMC Complementary and Alternative Medicine·Juraiporn SomboonwongMayuree H Tantisira
Dec 16, 2005·The Journal of Investigative Dermatology·Gys J de JonghJoost Schalkwijk
Jun 12, 2004·The Journal of Investigative Dermatology·Rita WongNicholas R Benson
May 13, 2008·Nanomedicine : Nanotechnology, Biology, and Medicine·Julio C Garay-JimenezEdward Turos
Jan 13, 2009·International Journal of Cosmetic Science·A OttoJ W Wiechers
May 7, 2002·Genomics·Fred van RuissenJoost Schalkwijk
May 28, 2016·The Journal of Pharmacy and Pharmacology·Katrien Van BocxlaerSimon L Croft
Jan 24, 2014·Experimental Dermatology·Kyung Ah CheongAi-Young Lee
Dec 5, 2017·Annals of Dermatology·Gwang Hoon KimAi-Young Lee
Jun 20, 2003·Journal of Biochemical and Molecular Toxicology·James V RogersJames N McDougal
Dec 25, 2009·Journal of Materials Science. Materials in Medicine·Ana GârleaM I Popa
Aug 10, 2005·Dermatology : International Journal for Clinical and Investigative Dermatology·Yutaka TakagiGenji Imokawa
Jan 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fred van RuissenJoost Schalkwijk
Feb 15, 2013·Chemical Society Reviews·Corine GérardinBénédicte Lebeau
Dec 3, 2016·The Review of Scientific Instruments·D PeevM Schubert
Apr 20, 2013·ACS Applied Materials & Interfaces·Carolina AduraMarcelo J Kogan

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