Differential effects of dimethyl sulfoxide and sodium butyrate on alpha-fetoprotein, albumin, and transferrin production by rat hepatomas in culture

In Vitro
H A SchutS S Thorgeirsson

Abstract

Radioimmunoassay was used to determine alpha-fetoprotein (AFP), albumin, and transferrin production (ng/10(5) cells/24 h) by two cell lines (7777 and 8994) derived from chemically induced rat hepatomas. alpha-Fetoprotein production was high (2000 to 4400) in 7777, but was very low (0.2 to 0.4) in 8994. Albumin production varied from 0.4-0.8 (7777) to 14-26 (8994). Both lines produced substantial amounts of transferrin (180 to 240 by 7777 and 29 to 42 by 8994). Addition of dimethyl sulfoxide (DMSO, 1 to 4%) or sodium butyrate (BA, 0.5 to 2.0 mM) to the medium inhibited growth in both lines, but 8994 was more sensitive to these agents than 7777. Dimethyl sulfoxide treatment (2 to 4%) resulted in a dose-related decrease (less than 10% of control at 4% DMSO) in AFP, albumin, and transferrin production by 7777, but in 8994, DMSO (1 to 2%) resulted in an increase (up to sixfold) in albumin and transferrin production, without affecting AFP production. By contrast, BA (2 to 4 mM) stimulated the production of all three proteins in both lines, most notably that of albumin (up to sixfold) by 7777 and that of AFP (up to 20-fold) by 8994. It is concluded that both DMSO and BA can enhance the expression of differentiated functions of the hep...Continue Reading

References

Feb 1, 1976·In Vitro·K N Prasad, P K Sinha
Jan 12, 1976·Biochemical and Biophysical Research Communications·B de NechaudV R Potter
Jun 20, 1977·Biochemical and Biophysical Research Communications·J B McMahonC J Smith
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H LeffertI Arias
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A E SiricaH C Pitot
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J M Sala-TrepatJ Bonner
Apr 13, 1979·Biochemical and Biophysical Research Communications·M P Leibovitch, J Kruh
Sep 1, 1979·Proceedings of the Society for Experimental Biology and Medicine·T S TralkaJ S Tseng
Oct 12, 1979·Biochemical and Biophysical Research Communications·D M Lilley, A R Berendt
Jan 1, 1971·Advances in Cancer Research·G I Abelev
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·C FriendT Sato
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

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Citations

Nov 1, 1988·Somatic Cell and Molecular Genetics·R C MifflinJ Papaconstantinou
Apr 1, 1993·The International Journal of Biochemistry·M A Lea
Dec 15, 1986·International Journal of Cancer. Journal International Du Cancer·P J Higgins
Aug 15, 1988·International Journal of Cancer. Journal International Du Cancer·M P Ryan, P J Higgins

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