Differential effects of erufosine on proliferation, wound healing and apoptosis in colorectal cancer cell lines

Oncology Reports
Ferda Kaleağasıoğlu, Martin R Berger

Abstract

The alkylphosphocholine, erucylphospho-N,N, N-trimethylpropanolamine (erufosine), has demonstrated anticancer effects in various cell lines, including leukemia, multiple myeloma, bladder, breast and oral squamous cell carcinoma cells. The purpose of the present study was to investigate its antiproliferative, antimigratory and pro-apoptotic effects in colorectal cancer cell lines, SW480 and CC531. The antiproliferative effect was determined by (3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) (MTT) dye reduction assay following exposure to erufosine (3.1-100 µM) for 24-72 h. The antimigratory effect of erufosine (1.6-6 µM) was investigated by a wound healing assay for 12-48 h. Caspase-3/-7 activity was measured to detect apoptotic cell death. Erufosine inhibited cell proliferation in a dose- and time-dependent manner. The IC50 values following 72 h of incubation were 3.4 and 25.4 µM for SW480 and CC531 cells, respectively. erufosine at concentrations of 50 and 100 µM induced caspase-3/-7 activity concentration-dependently in SW480 cells, but only at 100 µM in CC531 cells. Incubation of SW480 cells with erufosine (1.56 µM) for 48 h inhibited migration into the scratched area by 54% as compared to the untreated cells...Continue Reading

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Citations

Dec 25, 2015·International Journal of Oncology·George Mihai NitulescuAristidis M Tsatsakis
Oct 16, 2018·Anti-cancer Agents in Medicinal Chemistry·Ferda KaleağasıoğluMartin R Berger
Mar 31, 2017·Oncology Letters·Daniela Fortunato RêgoEliete Neves Silva Guerra
May 16, 2020·Frontiers in Pharmacology·Ferda KaleağasıoğluMartin R Berger
Mar 3, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiang-Yi YangRi-Su Na

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