Differential effects of fumonisin B1 on cell death in cultured cells: the significance of the elevated sphinganine

Archives of Pharmacal Research
C H YuH S Yoo

Abstract

Fumonisins are specific inhibitors of ceramide synthase in sphingolipid metabolism. An alteration in sphingolipid metabolism as a result of fumonisin exposure is related to cell death (Yoo et al., 1992). The objective of this study was to investigate whether elevated free sphinganine levels are related to the sensitivity of cultured cells to fumonisin exposure. Fumonisin B1 elevated the intracellular free sphinganine concentraions in both LLC-PK1 and Chinese hamster ovary (CHO) cells. However, CHO cells are resistant to fumonisin cytotoxicity at 50 microM, while LLC-PK1 cells are sensitive at concentrations greater than 35 microM. The intracellular concentration of free sphinganine in LLC-PK, cells treated at 50 microM fumonisin B1 for 72 h was approximately 1450 pmol/mg protein relative to the 37 pmol observed in the control culture. Under the same conditions, the population of apoptotic cells in the 50 M fumonisin B1-treated culture was approximately 37% of the total compared to 12% in the control. The caspase III-like activity after 72 h in the 50 microM fumonisin B1-exposed culture increased to approximately 50 pmol/mg protein/hr compared to 6 pmol/mg protein/hr in the control. L-cycloserine, a serine palmitoyltransferase i...Continue Reading

References

Jun 1, 1988·Analytical Biochemistry·A H MerrillD C Liotta
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jan 1, 1997·Toxicology and Applied Pharmacology·A H MerrillE Wang
Jan 7, 1999·Annals of Tropical Paediatrics·M G Nair
Apr 1, 1965·Journal of the American Oil Chemists' Society·R C GAVER, C C SWEELEY
Feb 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H S YooR T Riley

❮ Previous
Next ❯

Citations

Nov 25, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F MinerviniK M Flynn
Nov 26, 2002·The Plant Journal : for Cell and Molecular Biology·Stefka D SpassievaJacques Hille
Oct 25, 2006·Journal of Toxicology and Environmental Health. Part a·Dong-Hyun KimSeikwan Oh
Dec 8, 2004·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sarah S RentzRonald T Riley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis