Differential effects of heterochromatin protein 1 isoforms on mitotic chromosome distribution and growth in Dictyostelium discoideum

Eukaryotic Cell
Markus KallerWolfgang Nellen

Abstract

Heterochromatin protein 1 (HP1) is a well-characterized heterochromatin component conserved from fission yeast to humans. We identified three HP1-like genes (hcpA, hcpB, and hcpC) in the Dictyostelium discoideum genome. Two of these (hcpA and hcpB) are expressed, and the proteins colocalized as green fluorescent protein (GFP) fusion proteins in one major cluster at the nuclear periphery that was also characterized by histone H3 lysine 9 dimethylation, a histone modification so far not described for Dictyostelium. The data strongly suggest that this cluster represents the centromeres. Both single-knockout strains displayed only subtle phenotypes, suggesting that both isoforms have largely overlapping functions. In contrast, disruption of both isoforms appeared to be lethal. Furthermore, overexpression of a C-terminally truncated form of HcpA resulted in phenotypically distinct growth defects that were characterized by a strong decrease in cell viability. Although genetic evidence implies functional redundancy, overexpression of GFP-HcpA, but not GFP-HcpB, caused growth defects that were accompanied by an increase in the frequency of atypic anaphase bridges. Our data indicate that Dictyostelium discoideum cells are sensitive to c...Continue Reading

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May 13, 2006·Current Genetics·Heriberto Cerutti, J Armando Casas-Mollano
Mar 11, 2008·Eukaryotic Cell·Hui LiArturo De Lozanne
Sep 24, 2008·Molecular and Cellular Biology·Mahito SadaieJun-Ichi Nakayama
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May 19, 2011·Development, Growth & Differentiation·Michelle StevenseTetsuya Muramoto
Sep 2, 2016·Mobile DNA·Thomas SpallerThomas Winckler

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