Differential effects of insulin and insulin-like growth factor I on the production of plasma steroid-binding globulins by human hepatoblastoma-derived (Hep G2) cells

The Journal of Clinical Endocrinology and Metabolism
J C CraveM Pugeat

Abstract

Changes in the plasma levels of corticosteroid-binding globulin (CBG) and sex hormone-binding globulin (SHBG) from birth to adulthood suggest that growth factors might influence clearance and/or hepatic secretion of CBG and SHBG in humans. The effects of insulin-like growth factor I (IGF-I) and insulin on CBG and SHBG synthesis by a clone of human hepatoblastoma-derived (Hep G2) cell lines were therefore investigated. The results showed that the immunoconcentrations of CBG and SHBG, as well as total protein concentration in culture medium from Hep G2 cells, were decreased by IGF-I and insulin. However, although the CBG-to-total protein ratio was decreased dose dependently by IGF-I and insulin, IGF-I and insulin did not dose-dependently decrease the SHBG-to-total protein ratio. The steady state levels of CBG and SHBG messenger RNAs (mRNAs) were reduced dose dependently by IGF-I with a half-effect at 5.4 +/- 1.9 and 4.6 +/- 1.6 nmol/L, respectively, and by insulin with a half-effect at 4.3 +/- 1.1 and 4.3 +/- 1.4 nmol/L, respectively. The maximum inhibitory effect of IGF-I on CBG mRNA level was 48 +/- 17% of control values and 60 +/- 13% for SHBG mRNA level. The changes in CBG mRNA levels were quantitatively similar to the change...Continue Reading

Citations

Nov 10, 2007·The Journal of Clinical Investigation·David M SelvaGeoffrey L Hammond
May 3, 2008·Endocrine Reviews·Sundeep KhoslaEric Orwoll
Apr 7, 2009·Metabolic Syndrome and Related Disorders·Gabrielle Page-WilsonKathryn M Rexrode
Nov 26, 2009·The Journal of Clinical Endocrinology and Metabolism·Gail A LaughlinElizabeth Barrett-Connor
Jan 29, 2010·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Kishore M LakshmanAndre B Araujo
Jan 31, 2013·World Journal of Orthopedics·Antonio HerreraLuis Gracia
Dec 20, 2014·Hormones & Cancer·Joanne KotsopoulosSteven A Narod
Aug 15, 2012·Nature Reviews. Endocrinology·Ilias PerogamvrosPeter J Trainer
Dec 22, 2009·Endocrinology·Elodie M RichardMarie-Pierre Moisan
Jul 31, 2018·Journal of Diabetes Research·Mukhtar MohammedRiyadh Saif-Ali

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