Differential effects of intravenous anesthetics on ciliary motility in cultured rat tracheal epithelial cells

Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie
Hiroko IidaKazuhiko Fukuda

Abstract

It has been shown that airway ciliary function is impaired by several anesthetic or sedative drugs, which may predispose anesthetized or intensive care patients to respiratory complications, such as hypoxemia, atelectasis and pulmonary infection. We studied the effects of midazolam, propofol, dexmedetomidine, ketamine, fentanyl, thiopental and pentobarbital on ciliary beat frequency (CBF) in isolated and cultured rat tracheal epithelial (RTE) cells, to investigate their direct CBF action removing influences of non-epithelial cells. Rat tracheal epithelial cells were purely isolated from tracheas of adult male Sprague-Dawley rats. After 14 to 21 days of culture, the images of motile cilia were videotaped using a phase-contrast microscope. Baseline CBF and CBF 30 or 50 min after administration of vehicle or one of the above agents were computer-analyzed. Midazolam (0.3-10 microM), propofol (1-100 microM), dexmedetomidine (1-100 nM), fentanyl (0.1-10 nM) and thiopental (30-300 microM) had no effect on CBF. Ketamine at a supraclinical dose (1000 microM) increased CBF (22 +/- 13, mean +/- standard deviation, % increase from baseline; baseline = 100%) significantly (P < 0.01). Fentanyl at a high clinical dose (100 nM) increased CBF s...Continue Reading

References

Jan 1, 1975·Respiration; International Review of Thoracic Diseases·J Iravani, G N Melville
Mar 1, 1979·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·M KingP T Macklem
Sep 1, 1979·Anesthesia and Analgesia·A R Forbes, G Gamsu
Mar 1, 1977·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·G Patrick, C Stirling
May 1, 1975·Journal of Applied Physiology·J F LandaM I Lebeaux
Aug 1, 1992·Journal of Pharmacological and Toxicological Methods·L N Curtis, J L Carson
Apr 1, 1992·Thorax·A HasaniS W Clarke
Oct 1, 1990·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·P P PhillipsE B Kern
Jan 1, 1995·Methods in Cell Biology·L E OstrowskiP Nettesheim
Jun 1, 1993·In Vitro Cellular & Developmental Biology. Animal·L KaartinenS H Randell
Mar 1, 1995·American Journal of Respiratory Cell and Molecular Biology·A B ClarkL E Ostrowski
Feb 1, 1996·British Journal of Anaesthesia·D A SelwynJ A Langton
Dec 1, 1996·American Journal of Respiratory and Critical Care Medicine·A WannerT G O'Riordan
Dec 6, 1997·British Journal of Anaesthesia·J H Raphael, M W Butt
Jan 27, 1998·Acta Oto-laryngologica·M JohnstonA Drake-Lee
May 15, 1998·Anästhesiologie, Intensivmedizin, Notfallmedizin, Schmerztherapie : AINS·F KonradA Goertz
Dec 5, 2002·The Journal of Allergy and Clinical Immunology·Matthias Salathe
Mar 4, 2003·Journal of Applied Physiology·Lian WangDonovan B Yeates
Oct 23, 2003·Anesthesia and Analgesia·Masami SatoKazuhiko Fukuda
Jun 1, 1993·In Vitro Cellular & Developmental Biology. Animal·L KaartinenS H Randell

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Citations

Jan 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Mark J HoeggerMichael J Welsh
Feb 27, 2015·Frontiers in Pediatrics·Nils WelcheringCecilia W Lo
Nov 2, 2014·Frontiers in Pediatrics·Adam B ChristopherCecilia W Lo
Nov 16, 2016·Cells·Asma Yaghi, Myrna B Dolovich
Mar 14, 2014·The Lancet. Respiratory Medicine·Robert P DicksonGary B Huffnagle
Jul 18, 2019·Pediatric Pulmonology·Céline KempeneersMark A Chilvers
Mar 3, 2021·Scientific Reports·Kyle S FeldmanMaliha Zahid

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