Differential effects of n-3 and n-6 fatty acids on prostaglandin F2alpha production by bovine endometrial cells

Journal of Dairy Science
C Caldari-TorresL Badinga

Abstract

Recent studies have implicated n-3 polyunsaturated fatty acids in the reduction of eicosanoid production in the bovine uterus. The objective of this study was to determine whether the effect of eicosapentaenoic acid (EPA; C(20:5), n-3) on PGF2alpha production by bovine endometrial (BEND) cells is influenced by the quantity of linoleic acid (C(18:2), n-6) in the incubation medium. Confluent BEND cells were incubated in the absence (control) or presence of 100 microM of EPA for 24 h. After incubation, cells were rinsed and then stimulated with phorbol 12,13-dibutyrate (PDBu; 100 ng/mL) for 6 h. Additional sets of culture dishes were treated with a combination of EPA and increasing n-6/n-3 fatty acid ratios for 24 h and then challenged with PDBu for 6 h. The PDBu stimulated PGF2alpha secretion and upregulated steady-state concentrations of prostaglandin endoperoxide synthase-2 and peroxisome proliferator-activated receptor delta mRNA within 6 h. Preincubation of BEND cells with EPA for 24 h decreased PGF2alpha response to phorbol ester, but had no detectable effects on prostaglandin endoperoxide synthase-2 or peroxisome proliferator-activated receptor delta mRNA abundance in PDBu-stimulated BEND cells. The inhibitory effect of EPA...Continue Reading

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Citations

Dec 15, 2010·Equine Veterinary Journal·S Wilsher, W R Allen
Apr 19, 2018·Biology of Reproduction·Katrin GillerSusanne E Ulbrich
Jun 13, 2019·Veterinary Research Communications·Pamela ValenzuelaMaria A Hidalgo
Apr 28, 2018·Reproductive Biology and Endocrinology : RB&E·Virginie MaillardSebastien Elis

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