Differential effects of p38- and extracellular signal-regulated kinase mitogen-activated protein kinase inhibitors on inducible nitric oxide synthase and tumor necrosis factor production in murine macrophages stimulated with Streptococcus pneumoniae

The Journal of Infectious Diseases
Richelle M MonierB K English

Abstract

The role of p38- and extracellular signal-regulated kinase (ERK) mitogen-activated protein (MAP) kinase pathways in the up-regulation of inducible nitric oxide synthase (iNOS) and tumor necrosis factor (TNF) production in macrophages stimulated with Streptococcus pneumoniae was examined. Inhibitors of p38 kinases effected significant decreases in the accumulation of iNOS protein in macrophages challenged with pneumococcal cell wall preparations or antibiotic-killed pneumococci, even when added up to 6 h after bacterial challenge. In contrast, ERK pathway inhibitors failed to inhibit pneumococcus-induced iNOS protein accumulation. ERK pathway inhibitors significantly reduced TNF secretion when added at the same time as pneumococcal challenge, and inhibitors of both ERK and p38 pathways reduced TNF secretion when added to the macrophages 1 h before stimulation. These data confirm the importance of the p38 and ERK MAP kinase pathways in macrophage activation by bacterial products but indicate that these 2 kinase pathways regulate different macrophage responses in a temporally distinct manner.

Citations

Sep 14, 2007·Inflammation·Vardit RubovitchMoshe Kalina
Mar 5, 2004·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Sang Hyun KimRaghubir P Sharma
Mar 8, 2006·The Journal of Biological Chemistry·Adam J RatnerJeffrey N Weiser
Jul 5, 2005·American Journal of Respiratory Cell and Molecular Biology·Leif D NelinYusen Liu
May 17, 2008·American Journal of Respiratory Cell and Molecular Biology·Feng XuKlaus Dalhoff
Mar 11, 2015·American Journal of Respiratory Cell and Molecular Biology·Janine ZahltenStefan Hippenstiel
Apr 8, 2015·Research in Veterinary Science·Jennifer T BrisbinShayan Sharif

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