Differential effects of parathyroid hormone on protein phosphorylation in two osteoblastlike cell populations isolated from neonatal mouse calvaria

Calcified Tissue International
N S Krieger, T J Hefley

Abstract

To further understand the mechanism of PTH effects on bone and bone cells, we have analyzed the effect of PTH on specific protein phosphorylation in cells isolated from neonatal mouse calvaria. Four populations of cells (I-IV), isolated by sequential digestion with chromatographically purified bacterial collagenase isozymes and neutral proteinase, were cultured overnight. Alkaline phosphatase activity was greater than acid phosphatase activity in all four populations. PTH stimulated cyclic AMP production in all four populations, although the effect was greatest in populations II and III. Cultured cells were treated with PTH for up to 15 minutes. Cytosolic and membrane fractions were obtained and assayed for in vitro protein phosphorylation. No hormonal effects were found in membrane fractions. In cytosol fractions, treatment of the population II cells for 10-15 minutes with 0.1 microM PTH decreased the subsequent protein phosphorylation of an 85,000 Mr protein. In contrast, PTH treatment increased in vitro phosphorylation of both the 85,000 and 35,000 Mr proteins in population III cells. Phosphorylation of the 35,000 Mr protein was cyclic AMP-dependent. All of the phosphoproteins appeared to be phosphorylated solely on serine o...Continue Reading

References

Jan 13, 1978·Science·P Greengard
Apr 1, 1979·The Journal of Clinical Endocrinology and Metabolism·S R GoldringS M Krane
Dec 14, 1978·Biochemical and Biophysical Research Communications·A H Tashjian, L Levine
Dec 1, 1987·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T J Hefley
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R D Fleischmann, J M Pawelek
Jan 1, 1985·Annual Review of Biochemistry·T Hunter, J A Cooper
Aug 1, 1987·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P J Stewart, P H Stern
Apr 1, 1986·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M S Rappaport, P H Stern
Feb 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A A Cole, L M Walters
Jan 1, 1984·Methods in Enzymology·T M Martensen
Jan 1, 1981·Calcified Tissue International·G A Rodan, T J Martin
Jul 1, 1980·Endocrinology·L G RaiszE Canalis

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Citations

Sep 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N S Krieger
May 28, 2010·American Journal of Physiology. Renal Physiology·Kevin K Frick, David A Bushinsky
Jun 1, 2012·American Journal of Physiology. Renal Physiology·Nancy S KriegerDavid A Bushinsky
Nov 5, 2010·American Journal of Physiology. Renal Physiology·Nancy S Krieger, David A Bushinsky
Feb 18, 2016·Kidney International·Nancy S KriegerDavid A Bushinsky
Mar 14, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nancy S KriegerDavid A Bushinsky
Mar 17, 2017·American Journal of Physiology. Renal Physiology·Nancy S Krieger, David A Bushinsky
Jun 17, 2004·Current Opinion in Nephrology and Hypertension·Nancy S KriegerDavid A Bushinsky
Nov 30, 2000·American Journal of Physiology. Renal Physiology·N S KriegerD A Bushinsky
Aug 17, 2021·American Journal of Physiology. Renal Physiology·Nancy S Krieger, David A Bushinsky

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