Differential effects of PDGF-BB on matrix metalloproteases and cytokine release in fibroblasts of Type 2 diabetic patients and normal controls in vitro

Journal of Diabetes and Its Complications
Ralf LobmannHendrik Lehnert

Abstract

The complex process of wound healing is regulated by various growth factors. The systemic character of diabetes mellitus favors the chronification of diabetic wounds. In this study, the in vitro effects of platelet-derived growth factor (PDGF)-BB on the expression of cytokines and matrix metalloproteases (MMPs) in fibroblasts of Type 2 diabetic patients and healthy controls were investigated. We studied six Type 2 diabetic patients (mean Hba1(c)=7.5%) and six healthy controls. For proliferation studies, cultivated fibroblasts, prepared from biopsies taken from the thigh, were stimulated with different concentrations of PDGF. After 48 h, the expression of MMPs and cytokines was measured. We analysed the mRNA expression by RT-PCR (TaqMan), tissue protein levels by zymography, and cell supernatant levels by ELISA. Levels of MMP-mRNA were elevated in diabetic fibroblasts compared with healthy controls. At baseline, MMP-2 protein levels were significantly increased in the fibroblast of diabetic patients (P=.019). For MMP-9, a trend towards higher levels (P=.3) was found. After incubation with PDGF, a significant reduction of MMP-9 (P=.01) and MMP-13 (P=.04) was found. Analysis of cytokine release in cell culture supernatant showed e...Continue Reading

References

Apr 1, 1991·Journal of Cellular Biochemistry·G F PierceA Thomason
Apr 1, 1991·Journal of Cellular Biochemistry
Jan 1, 1987·Journal of Ocular Pharmacology·N L Burstein
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S E LynchH N Antoniades
Oct 1, 1994·Current Opinion in Cell Biology·J Gailit, R A Clark
Feb 1, 1995·The Journal of Investigative Dermatology·E C BullenE W Howard
Jul 1, 1994·The Journal of Clinical Investigation·U K Saarialho-KereH G Welgus
Jul 1, 1993·The Journal of Investigative Dermatology·A B WysockiF Grinnell
Jul 1, 1996·The Journal of Trauma·D G Greenhalgh
Jul 1, 1996·The British Journal of Dermatology·M VaalamoU K Saarialho-Kere
Aug 1, 1997·The Journal of Investigative Dermatology·H D BeerS Werner
Feb 5, 1998·Experimental Dermatology·V M Kähäri, U Saarialho-Kere
Oct 20, 1998·American Journal of Surgery·T J Wieman
Feb 3, 1999·The Journal of Surgical Research·B C NwomehD R Yager
Sep 2, 1999·The New England Journal of Medicine·A J Singer, R A Clark
Jan 13, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·N J TrengoveG Schultz
Apr 12, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·N J TrengoveM C Stacey
Sep 22, 2000·International Journal of Molecular Medicine·L Ravanti, V M Kähäri
Nov 18, 2000·Arthritis and Rheumatism·Y ShigeyamaS Gay
Feb 24, 2001·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·R H LeeA Barbul
Mar 15, 2001·American Journal of Surgery·W G PayneM C Robson
Mar 27, 2001·Connective Tissue Research·J F WangD A Hart
Apr 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E R FedykT A Springer
Jan 18, 2002·Journal of the American Podiatric Medical Association·David G Armstrong, Edward B Jude
May 2, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Glenn P LadwigGregory S Schultz
Oct 18, 2002·Journal of Cellular Physiology·K E MoyerH P Ehrlich
Jan 29, 2005·Diabetes Care·Ralf LobmannHendrik Lehnert
Oct 1, 1996·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·B A Mast, G S Schultz

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Citations

Mar 30, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hye Sung Kim, Hyuk Sang Yoo
Jan 29, 2008·Seminars in Arthritis and Rheumatism·Y Stephanie GuM Eric Gershwin
Jan 22, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Robert M CrewsWarren O Haggard
Jul 25, 2012·Journal of the American Podiatric Medical Association·Xiao-Ying XieLi Yan
Jul 21, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Melpomeni PeppaSotirios A Raptis
Apr 26, 2012·Biological Research for Nursing·Takashi SugimotoHiromi Sanada
Feb 21, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Satish PatelDeependra Singh
Jun 21, 2007·Cirugía española·José Luis Lázaro-MartínezFrancisco Javier Aragón-Sánchez

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