PMID: 11897856Mar 19, 2002Paper

Differential effects of sarcoplasmic reticular Ca(2+)-ATPase inhibition on charge movements and calcium transients in intact amphibian skeletal muscle fibres

The Journal of Physiology
Sangeeta ChawlaChristopher L-H Huang

Abstract

A hypothesis in which intramembrane charge reflects a voltage sensing process allosterically coupled to transitions in ryanodine receptor (RyR)-Ca(2+) release channels as opposed to one driven by release of intracellularly stored Ca(2+) would predict that such charging phenomena should persist in skeletal muscle fibres unable to release stored Ca(2+). Charge movement components were accordingly investigated in intact voltage-clamped amphibian fibres treated with known sarcoplasmic reticular (SR) Ca(2+)-ATPase inhibitors. Cyclopiazonic acid (CPA) pretreatment abolished Ca(2+) transients in fluo-3-loaded fibres following even prolonged applications of caffeine (10 mM) or K(+) (122 mM). Both CPA and thapsigargin (TG) transformed charge movements that included delayed (q(gamma)) "hump" components into simpler decays. However, steady-state charge-voltage characteristics were conserved to values (maximum charge, Q(max) approximately equal to 20-25 nC microF(-1); transition voltage, V* approximately equal to -40 to-50 mV; steepness factor, k approximately equal to 6-9 mV; holding voltage -90 mV) indicating persistent q(gamma) charge. The features of charge inactivation similarly suggested persistent q(beta) and q(gamma) charge contrib...Continue Reading

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Citations

Sep 7, 2006·Pflügers Archiv : European journal of physiology·Juliet A Usher-SmithChristopher L-H Huang
Jan 29, 2008·Journal of Muscle Research and Cell Motility·Nazira Píriz, Gonzalo Pizarro
Dec 9, 2003·The Journal of Physiology·Roberta SqueccoFabio Francini
Jul 3, 2010·Journal of Muscle Research and Cell Motility·J Fernando Olivera, Gonzalo Pizarro
Oct 14, 2011·Journal of Muscle Research and Cell Motility·Christopher L-H HuangJames A Fraser

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