PMID: 7335102Dec 1, 1981Paper

Differential effects of sulphur mustard on S-phase cells of primary fibroblast cultures from Syrian hamsters

Mutation Research
J R Savage, G Breckon

Abstract

Sulphur mustard (SM) (5 x 10(-8) M) given to primary Syrian hamster fibroblasts growing short-term in vitro produces a very sharp peak of chromatid aberrations 12-16 h after treatment. The composition of this peak has been investigated in relation to the cell cycle using the facility to divide S-phase into 5 cytologically defined sub-phases on the basis of replication band patterns following bromodeoxyuridine incorporation. It is shown that: (1) Considerable delay and perturbation of the cycle is present. (2) A major contribution to the aberration peak comes from pre-S cells, and the highest aberration frequencies are observed in such cells. (3) The bulk of the contribution from S-cells comes from the last subphase, SkV. (4) The majority of early S cells (sub-phases SkI-IV) fail to reach division within 36 h. Of the total S-cells scored, 54% are non-SkV in controls but only 14% after SM. (5) Aberrations are localized to late-replicating regions in SkV but are random in pre-S. (6) No measurable perturbation of replication programme was found in chromosome arms synthesizing in late SkV after SM. (7) The rapid fall in aberration frequency at later sampling times is consistent with a quick and efficient repair of DNA lesions which ...Continue Reading

References

Apr 1, 1976·Journal of Medical Genetics·J R Savage
Jan 1, 1975·Cytogenetics and Cell Genetics·M A KimK H Grzeschik
Jul 22, 1969·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·H J Evans, D Scott
Jun 8, 1968·Nature·J J RobertsT P Brent
Jan 1, 1980·Cytogenetics and Cell Genetics·J R SavageT R Bigger

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Citations

Apr 1, 1991·Trends in Pharmacological Sciences·U Wormser
May 1, 1997·Survey of Ophthalmology·Y SolbergM Belkin
Jun 1, 1984·Journal of Medical Genetics·J R Savage, R Prasad
Nov 1, 1992·Environmental Health Perspectives·A P Watson, G D Griffin
Apr 16, 2014·Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis·Swetha InturiRajesh Agarwal
May 1, 1993·International Journal of Immunopharmacology·M Ebtekar, Z M Hassan
Jul 28, 2011·The Ocular Surface·Alireza Baradaran-RafiiScheffer C G Tseng
Feb 19, 2011·Critical Reviews in Toxicology·Kamyar GhabiliMohammadali M Shoja

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