Differential effects of superoxide dismutase and superoxide dismutase/catalase mimetics on human breast cancer cells

Breast Cancer Research and Treatment
Manisha H ShahHitesh M Peshavariya

Abstract

Reactive oxygen species (ROS) such as superoxide and hydrogen peroxide (H2O2) have been implicated in development and progression of breast cancer. In the present study, we have evaluated the effects of the superoxide dismutase (SOD) mimetic MnTmPyP and the SOD/catalase mimetic EUK 134 on superoxide and H2O2 formation as well as proliferation, adhesion, and migration of MCF-7 and MDA-MB-231 cells. Superoxide and H2O2 production was examined using dihydroethidium and Amplex red assays, respectively. Cell viability and adhesion were measured using a tetrazolium-based MTT assay. Cell proliferation was determined using trypan blue assay. Cell cycle progression was analyzed using flow cytometry. Clonal expansion of a single cell was performed using a colony formation assay. Cell migration was measured using transwell migration assay. Dual luciferase assay was used to determine NF-κB reporter activity. EUK 134 effectively reduced both superoxide and H2O2, whereas MnTmPyP removed superoxide but enhanced H2O2 formation. EUK 134 effectively attenuated viability, proliferation, clonal expansion, adhesion, and migration of MCF-7 and MDA-MB-231 cells. In contrast, MnTmPyP only reduced clonal expansion of MCF-7 and MDA-MB-231 cells but had ...Continue Reading

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Citations

Dec 8, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Arunkumar ArumugamRajkumar Lakshmanaswamy
Jun 29, 2015·Free Radical Biology & Medicine·Christophe GlorieuxPedro Buc Calderon
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Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lozan TodorovIrena Kostova

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