Differential effects of temperature on natural transformation to erythromycin and nalidixic acid resistance in Campylobacter coli.

Applied and Environmental Microbiology
Joo-Sung KimS Kathariou

Abstract

Campylobacter jejuni and Campylobacter coli are naturally competent, but limited information exists on the impact of environmental conditions on transformation. In this study, we investigated the impact of temperature and microaerobic versus aerobic atmosphere on transformation of C. coli to erythromycin and nalidixic acid resistance. Frequency of transformation was not significantly different between microaerobic (5 to 10% CO(2)) and aerobic conditions. However, C. coli was transformed to erythromycin resistance at a significantly higher frequency at 42 degrees C than at 25 degrees C (P < 0.05), and few or no transformants were obtained at 25 degrees C. In contrast, transformation to nalidixic acid resistance was highly efficient at both 42 degrees C and 25 degrees C and was similar or, at the most, fourfold higher at 42 degrees C than at 25 degrees C. DNase I treatment experiments suggested that steps both prior and subsequent to internalization of DNA were influenced by temperature in the case of transformation of C. coli to erythromycin resistance. However, the moderately increased (fourfold) frequency of transformation to nalidixic acid resistance at 42 degrees C compared to that at 25 degrees C was exclusively associated ...Continue Reading

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Citations

Mar 13, 2009·Foodborne Pathogens and Disease·Peter Gerner-Smidt, Jean M Whichard
Oct 11, 2012·PloS One·Christina S VeggeHanne Ingmer
Sep 14, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Ole Herman AmburDaniel E Rozen
Apr 16, 2017·Applied and Environmental Microbiology·Hannah Bolinger, Sophia Kathariou
Nov 8, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Harris BernsteinRichard E Michod
Oct 15, 2021·Frontiers in Microbiology·Vanina Guernier-CambertTorey Looft

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