Differential effects of the mismatch repair genes MSH2 and MSH3 on homeologous recombination in Saccharomyces cerevisiae

Molecular & General Genetics : MGG
E M SelvaR Lahue

Abstract

The products of the yeast mismatch repair genes MSH2 and MSH3 participate in the inhibition of genetic recombination between homeologous (divergent) DNA sequences. In strains deficient for these genes, homeologous recombination rates between repeated elements are elevated due to the loss of this inhibition. In this study, the effects of these mutations were further analyzed by quantitation of mitotic homeologous recombinants as crossovers, gene conversions or exceptional events in wild-type, msh2, msh3 and msh2 msh3 mutant strains. When homeologous sequences were present as a direct repeat in one orientation, crossovers and gene conversions were elevated in msh2, msh3 and msh2 msh3 strains. The increases were greater in the msh2 msh3 double mutant than in either single mutant. When the order of the homeologous sequences was reversed, the msh2 mutation again yielded increased rates of crossovers and gene conversions. However, in an msh3 strain, gene conversions occurred at higher levels but interchromosomal crossovers were not increased and intrachromosomal crossovers were reduced relative to wild type. The msh2 msh3 double mutant behaved like the msh2 single mutant in this orientation. Control strains harboring homologous dupli...Continue Reading

Citations

Aug 1, 2000·Mutation Research·B D Harfe, S Jinks-Robertson
Mar 20, 2002·Biochimie·Sandrine Jacob, Françoise Praz
Apr 8, 2000·Current Opinion in Genetics & Development·J Jiricny, M Nyström-Lahti
Jun 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Neal SugawaraJames E Haber
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·H Flores-Rozas, R D Kolodner
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·C Flores, W Engels
Jun 9, 2011·BMC Evolutionary Biology·Yevgeniy RaynesPaul D Sniegowski
Jan 8, 2016·DNA Repair·Khek-Chian ThamJoyce H G Lebbink
Apr 28, 2004·FEMS Microbiology Reviews·U Klinner, B Schäfer
Nov 13, 2012·PLoS Genetics·Diana D VillarrealSang Eun Lee
Sep 4, 2010·Annals of Pediatric Cardiology·Sachin TalwarBalram Airan
Jun 23, 2001·Molecular Biology and Evolution·E D Ladoukakis, E Zouros
Mar 23, 2002·Genome Génome / Conseil National De Recherches Canada·Chongmei DongPeter Langridge
Mar 2, 2006·The Plant Journal : for Cell and Molecular Biology·Liangliang LiFrançois Belzile

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.