Differential effects of TNF-α and IL-1β on the control of metal metabolism and cadmium-induced cell death in chronic inflammation

PloS One
Paola BonaventuraPierre Miossec

Abstract

Interleukin-1-beta (IL-1β) and tumour necrosis factor-alpha (TNF-α) are both monocyte-derived cytokines. Both cytokines have been previously described to exert a role in rheumatoid arthritis (RA) pathogenesis synergizing with other pro-inflammatory mediators, such as interleukin-17 (IL-17) on target cells, for the perpetuation of the inflammatory response (e.g. IL-6 production). In the context of experimental RA, Cd addition has an anti-proliferative and anti-inflammatory effect when associated to IL-17/TNF-α stimulation, due to its accumulation in synoviocytes. The aim of this work was to evaluate if IL-1β interaction with IL-17 also contributes to metal-import mechanisms and its effects on cell viability and inflammation. IL-17 and IL-1β were added to synoviocyte cultures with or without exogenous Cd addition (0.1 ppm, 0.89 μM). IL-6 production, Cd import kinetics, gene expression of ZIP-8 importer and metallothioneins (MTs) and cell viability were evaluated by ELISA, inductively-coupled mass spectrometry (ICP-MS), q-RT-PCR and viability assays (neutral red and annexin V) respectively. IL-17 and IL-1β acted in synergy on synoviocytes to induce IL-6 production similarly to the IL-17/TNF-α combination. Metal import was lower wi...Continue Reading

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Feb 6, 2016·PloS One·Paola BonaventuraPierre Miossec

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Citations

Jan 8, 2020·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Baoyue ZhangMeng Jin
Aug 6, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Amrita K CheemaNasser M Rizk
Oct 24, 2020·Toxicology and Industrial Health·J Fernández-TorresK Martínez-Flores
Jun 10, 2021·Revista Portuguesa De Cardiologia : Orgão Oficial Da Sociedade Portuguesa De Cardiologia = Portuguese Journal of Cardiology : an Official Journal of the Portuguese Society of Cardiology·Hung-Chen LinPao-Hsien Chu
Jul 12, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yunsong JiangJihong Wu
Aug 17, 2021·Revista Portuguesa De Cardiologia·Hung-Chen LinPao-Hsien Chu

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Methods Mentioned

BETA
ELISA
PCR
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