Differential effects of vascular endothelial growth factor-C and placental growth factor-1 on the hydraulic conductivity of frog mesenteric capillaries

Journal of Vascular Research
N J HillmanDavid Bates

Abstract

Vascular endothelial growth factors (VEGFs) are known to increase vascular permeability. VEGF-A acts on two receptor tyrosine kinases, VEGF receptor-1 (VEGF-R1 or flt-1) and VEGF receptor-2 (VEGF-R2, flk-1 or KDR). VEGF-C acts only on VEGF-R2 on vascular endothelial cells, whereas placental growth factor-1 (PlGF-1) acts only on VEGF-R1. The effects of perfusion of these receptor-specific proteins on hydraulic conductivity (L(p)) was measured in frog mesenteric capillaries. The effect of PlGF on L(p) was not conclusive, and overall fluid flux did not increase during that time. VEGF-C acutely and transiently increased L(p) (4.5 +/- 0.9-fold), which was more obvious in a subset of vessels, in a similar manner to that reported for VEGF-A. In the subset of vessels in which VEGF-C significantly increased L(p) acutely, there was a sustained 12-fold increase in L(p) 20 min after perfusion, but this was not seen in those vessels which did not respond acutely to VEGF-C, or in vessels exposed to PlGF-1. L(p) was also increased 24 h after perfusion with VEGF-C, but not with PlGF-1. Western blot analysis showed that VEGF-R1 and VEGF-R2 are both present in frog tissue. These data show that the VEGFs that stimulate VEGF-R2 chronically increas...Continue Reading

Citations

Jun 10, 2006·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·M VölckerF Ziemssen
May 16, 2003·Vascular Pharmacology·D O Bates, S J Harper
Mar 29, 2002·Kidney International·Steven J HarperDavid O Bates
Jul 1, 2006·Lymphatic Research and Biology·K D JooryD O Bates
Apr 20, 2010·Cardiovascular Research·David O Bates
Aug 7, 2010·Journal of the American Society of Nephrology : JASN·Yan QiuSteve J Harper
Oct 27, 2004·Microvascular Research·Lucas DeMaioJohn M Tarbell
Jun 29, 2004·Microvascular Research·Bingmei M Fu, Shang Shen
Nov 23, 2011·Biochemical Society Transactions·David O Bates
Dec 17, 2002·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·C E WhittlesD O Bates
May 4, 2005·The International Journal of Lower Extremity Wounds·David O Bates, Rowan O Pritchard Jones
Nov 6, 2003·Journal of Orthopaedic Trauma·Bhaloo J DesaiRalph A Meyer
Jan 6, 2006·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Catherine A GlassDavid O Bates
Oct 11, 2003·American Journal of Physiology. Heart and Circulatory Physiology·T M PocockD O Bates
Feb 1, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Bingmei M Fu, Shang Shen
Apr 3, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Jerome W BreslinMack H Wu
Jun 15, 2011·BJOG : an International Journal of Obstetrics and Gynaecology·V L BillsD O Bates
Jul 10, 2003·The Journal of Bone and Joint Surgery. American Volume·Ralph A MeyerPatricia Garges
Jan 4, 2003·American Journal of Physiology. Heart and Circulatory Physiology·C A Glass, D O Bates

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.