Differential effects of vinblastine on polymerization and dynamics at opposite microtubule ends.

The Journal of Biological Chemistry
D PandaL Wilson

Abstract

We have characterized the effects of vinblastine on the growing and shortening dynamics at opposite ends of individual bovine brain microtubules at steady state in vitro by video microscopy. Vinblastine exerted strikingly different effects on the dynamics and polymer mass at the plus and minus ends of microtubules. At concentrations between 0.1 and 0.4 microM, the drug strongly depolymerized microtubules at minus ends, whereas it did not significantly depolymerize microtubules at plus ends. Vinblastine stabilized plus ends by suppressing the rate and extent of growth and shortening, decreasing the catastrophe frequency, and increasing the rescue frequency. In contrast, vinblastine destabilized minus ends by increasing the catastrophe frequency and decreasing the rescue frequency, whereas it had no effect on the rate or extent of growth or shortening. Thus, vinblastine moderately increased the overall dynamicity at minus ends while strongly suppressing dynamicity at plus ends. Both the kinetic destabilization of microtubules at minus ends and the stabilization at plus ends may contribute to the altered function of mitotic spindle microtubules of cells blocked in mitosis by low concentrations of vinblastine.

References

Jan 1, 1978·The Journal of Cell Biology·G B PiersonR H Himes
Oct 1, 1991·Pharmacology & Therapeutics·E K Rowinsky, R C Donehower
Jan 1, 1991·Pharmacology & Therapeutics·R F Luduena, M C Roach
Jan 1, 1989·International Review of Cytology·M F Carlier
Mar 1, 1986·The Journal of Cell Biology·E Schulze, M Kirschner
Jun 30, 1987·Biochemistry·E T O'BrienH P Erickson
Jan 5, 1986·Journal of Molecular Biology·M A JordanL Wilson
Dec 1, 1984·The Journal of Cell Biology·W M SaxtonJ R McIntosh
Nov 15, 1984·Nature·T Mitchison, M Kirschner
Oct 29, 1981·Nature·R L Margolis, L Wilson
Jul 25, 1982·Journal of Molecular Biology·L WilsonR L Margolis
Sep 25, 1982·Journal of Molecular Biology·V Prakash, S N Timasheff
Aug 1, 1995·The Journal of Cell Biology·B Mickey, J Howard
Jan 1, 1995·The Journal of Cell Biology·Y H Song, E Mandelkow
Feb 16, 1995·Nature·X Li, R B Nicklas
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M A JordanL Wilson
Jan 1, 1993·Cell Motility and the Cytoskeleton·R J Kowalski, R C Williams
Sep 1, 1995·Molecular Biology of the Cell·R DhamodharanP Wadsworth
Jan 1, 1996·Cell Motility and the Cytoskeleton·C S Spittle, L Cassimeris

❮ Previous
Next ❯

Citations

Jun 19, 2013·Chromosoma·Anna-Leena Salmela, Marko J Kallio
Mar 4, 1998·Current Opinion in Cell Biology·M A Jordan, L Wilson
Oct 30, 2001·Critical Reviews in Oncology/hematology·A Kruczynski, B T Hill
Feb 27, 1999·Molecular Microbiology·P DuwatA Gruss
May 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Renu MohanAnna Akhmanova
Sep 26, 2009·The Journal of Biological Chemistry·Zhiqing C ZhuHolly V Goodson
Feb 6, 2010·Journal of Medicinal Food·Vy NguyenGeorge Ayoub
Oct 6, 1998·Annual Review of Biochemistry·A Jordan, P Reichard
Jun 8, 2006·Annual Review of Biochemistry·Pär Nordlund, Peter Reichard
Aug 21, 2003·Breast Cancer Research : BCR·James H DozierSharon Lobert
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·D PandaL Wilson
Apr 5, 2011·Annual Review of Biochemistry·Joseph A Cotruvo, Joanne Stubbe
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·D PandaL Wilson
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·D PandaL Wilson
Nov 20, 2015·Environmental and Molecular Mutagenesis·Francesco MarchettiAntonella Russo
Aug 23, 2007·Journal of Pharmaceutical Sciences·David M Drake, Daniel W Pack
Jul 30, 2011·Journal of Molecular Graphics & Modelling·Evan B KellyM Klobukowski
Jun 9, 2004·European Journal of Biochemistry·Casper M JørgensenJan Martinussen
Mar 8, 2011·Medicinal Research Reviews·Richard A StantonRitu Aneja
Mar 7, 2002·Chemico-biological Interactions·Antonella MigliettaLudovica Gabriel
Aug 31, 2000·Annual Review of Biochemistry·E Nogales
Mar 3, 2007·Journal of Neuroscience Methods·Yanping Yan, Kendal Broadie
Sep 7, 2001·Annual Review of Microbiology·D Ritz, J Beckwith
Jul 10, 2001·Annual Review of Biophysics and Biomolecular Structure·E Nogales
Mar 18, 2017·International Journal of Molecular Sciences·Thazin Nwe AungDavid L Adelson
Mar 17, 2017·Molecular Biology of the Cell·Brian T CastleDavid J Odde
Jun 25, 2015·Drug Target Insights·Ihsan N ZulkipliAdi Idris
Sep 19, 2002·The Journal of Biological Chemistry·Cori N NewtonLeslie Wilson
Dec 31, 2005·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Pankaj Kumar MishraDebashish Chowdhury
May 1, 1999·The Journal of Biological Chemistry·S RoychowdhuryM M Rasenick
Nov 30, 2019·Current Pharmaceutical Biotechnology·Anand ThirupathiSampathkumar Natarajan
Dec 22, 1999·The American Journal of Physiology·P G Lloyd, C D Hardin

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.