Differential effects of Vpr on single-cycle and spreading HIV-1 infections in CD4+ T-cells and dendritic cells.

PloS One
Suresh de SilvaLi Wu

Abstract

The Vpr protein of human immunodeficiency virus type 1 (HIV-1) contributes to viral replication in non-dividing cells, specifically those of the myeloid lineage. However, the effects of Vpr in enhancing HIV-1 infection in dendritic cells have not been extensively investigated. Here, we evaluated the role of Vpr during infection of highly permissive peripheral blood mononuclear cells (PBMCs) and CD4(+) T-cells and compared it to that of monocyte-derived dendritic cells (MDDCs), which are less susceptible to HIV-1 infection. Infections of dividing PBMCs and non-dividing MDDCs were carried out with single-cycle and replication-competent HIV-1 encoding intact Vpr or Vpr-defective mutants. In contrast to previous findings, we observed that single-cycle HIV-1 infection of both PBMCs and MDDCs was significantly enhanced in the presence of Vpr when the viral stocks were carefully characterized and titrated. HIV-1 DNA quantification revealed that Vpr only enhanced the reverse transcription and nuclear import processes in single-cycle HIV-1 infected MDDCs, but not in CD4(+) T-cells. However, a significant enhancement in HIV-1 gag mRNA expression was observed in both CD4(+) T-cells and MDDCs in the presence of Vpr. Furthermore, Vpr comple...Continue Reading

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Citations

Feb 22, 2013·The Journal of Biological Chemistry·Suresh de SilvaLi Wu
Nov 20, 2013·Retrovirology·Michael D NekorchukCarlos M C de Noronha
Dec 17, 2014·Retrovirology·Marie-Lise BlondotFlorence Margottin-Goguet
Mar 15, 2015·AIDS Research and Human Retroviruses·Corine St GelaisLi Wu
Apr 27, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hichem LahouassaFlorence Margottin-Goguet
Apr 21, 2017·Journal of Virology·Caitlin M MillerSuryaram Gummuluru
Sep 25, 2015·Journal of Virology·Ferdinand RoeschOlivier Schwartz
Jan 12, 2017·International Journal of Molecular Sciences·María Eugenia González
Nov 8, 2018·Journal of Neurovirology·Dorota Piekna-PrzybylskaSanjay B Maggirwar
Oct 17, 2019·Frontiers in Immunology·Kirstie Melissa BertramAndrew Nicholas Harman
Sep 22, 2021·Nature Communications·Qiong ZhangTariq M Rana

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Methods Mentioned

BETA
transfection
ELISA
PCR
enzyme-linked immunosorbent assay

Software Mentioned

Excel

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