Differential effects of zinc binding on structured and disordered regions in the multidomain STIL protein

Chemical Science
Hadar AmartelyAssaf Friedler

Abstract

Binding of metal ions is an important regulatory mechanism in proteins. Specifically, Zn2+ binding to disordered regions commonly induces a disorder to order transition and gain of structure or oligomerization. Here we show that simultaneous binding of Zn2+ ions has different effects on structured and disordered domains in the same multidomain protein. The centrosomal STIL protein bound Zn2+ ions via both its structured N-terminal domain (NTD) and disordered central region (IDR). Zn2+ binding induced structural rearrangement of the structured NTD but promoted oligomerization of the IDR. We suggest that by binding Zn2+ STIL acquires a different conformation, which allows its oligomerization and induces its activity. Sequence alignment of the oligomerization region revealed a new suggested motif, SxKxS/SxHxS/SxLxS, which may participate in STIL oligomerization. Binding of the same metal ion through a disordered and a structured domain in the same protein is a property that may have implications in regulating the protein activity. By doing so, the protein achieves two parallel outcomes: structural changes and oligomerization that can take place together. Our results describe a new important role of the delicate interplay between s...Continue Reading

References

Jan 1, 1997·Folding & Design·A FontanaM Zambonin
Feb 21, 1998·Nucleic Acids Research·S HenikoffJ G Henikoff
Sep 8, 2000·The Journal of Biological Chemistry·U JakobJ C Bardwell
May 9, 2001·Biochimica Et Biophysica Acta·T MoldoveanuP L Davies
Dec 7, 2002·Nature Genetics·Sridhar RamaswamyTodd R Golub
Jan 21, 2004·Journal of Molecular Recognition : JMR·Matthew J Cliff, John E Ladbury
Aug 27, 2004·Journal of Molecular Biology·Hyung-Sik WonH Jane Dyson
Sep 10, 2004·The Journal of Biological Chemistry·Martine TourbezConstantin T Craescu
Jul 19, 2005·Molecular and Cellular Biology·Stefano CampanerIlan R Kirsch
Apr 26, 2007·Cancer Research·Ayelet ErezShai Izraeli
Jun 20, 2007·Molecular and Cellular Biology·Kathleen L PfaffLeonard I Zon
Nov 22, 2008·Biochemical Society Transactions·Jin LuClaudia A Blindauer
Feb 14, 2009·American Journal of Human Genetics·Arun KumarSusan H Blanton
Apr 28, 2009·The Journal of Biological Chemistry·Nathalie SelevsekHans W Adolph
Feb 2, 2010·Biochimica Et Biophysica Acta·Vladimir N Uversky, A Keith Dunker
Feb 4, 2010·The Journal of Cell Biology·Naomi R StevensJordan W Raff
Nov 4, 2010·Biophysical Journal·Timo BundDariush Hinderberger
Jan 20, 2011·Journal of Cell Science·Asher CastielShai Izraeli
Mar 16, 2011·Current Protein & Peptide Science·X Arias-MorenoA Velazquez-Campoy
Apr 2, 2011·The Journal of Biological Chemistry·Ana Cristina Sotomayor-PérezAlexandre Chenal
Feb 22, 2012·Journal of Cell Science·Christian ArquintErich A Nigg
Feb 22, 2012·Journal of Cell Science·Julia VulprechtAlwin Krämer
Jul 18, 2012·Cell Division·Karin Habermann, Bodo Mh Lange
Jan 18, 2013·PloS One·Florence GuillièreJ Iñaki Guijarro
Jan 22, 2013·Current Biology : CB·Gražvydas LukinavičiusKai Johnsson
Feb 14, 2013·International Journal of Biological Macromolecules·Ting YuShaoning Yu
Mar 9, 2013·Bioscience, Biotechnology, and Biochemistry·Ran-Hui LiYi-Zhi Zheng
May 9, 2013·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Audrey LabrieElisabeth Maurer-Spurej
Sep 12, 2013·Chemical Communications : Chem Comm·Hadar AmartelyAssaf Friedler
May 24, 2014·The Journal of Biological Chemistry·Magnus C MayerGerhard Multhaup
May 30, 2014·Accounts of Chemical Research·Peter FallerGiovanni La Penna
Jul 19, 2015·ELife·Christian ArquintTimm Maier

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
isothermal titration calorimetry
dynamic light scattering
peptide array
size exclusion chromatography
Light Scattering

Software Mentioned

Block Maker

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.