Differential endocrine regulation of genes enriched in initial segment and distal caput of the mouse epididymis as revealed by genome-wide expression profiling

Biology of Reproduction
Petra SipiläMatti Poutanen

Abstract

We have performed genome-wide expression profiling of endocrine regulation of genes expressed in the mouse initial segment (IS) and distal caput of the epididymis by using Affymetrix microarrays. The data revealed that of the 15 020 genes expressed in the epididymis, 35% were enriched in one of the two regions studied, indicating that differential functions can be attributed to the IS and the more distal caput regions. The data, furthermore, showed that 27% of the genes expressed in the IS and/or distal caput epididymidis are under the regulation of testicular factors present in the duct fluid, while bloodborne androgens can regulate for 14% of them. This is in line with the high testis dependency of epididymal physiology. We then focused on genes with moderate or strong expression, showing strict segment enrichment and strong dependency on testicular factors. Analyses of the 59 genes, including upregulated and downregulated genes, fulfilling the criteria indicated that the expression of 18 (17 downregulated genes; 1 upregulated gene) of 19 gonadectomy-responsive genes enriched in the IS was not maintained by the androgen treatment, whereas the expression of all six downregulated genes enriched in the distal caput and the major...Continue Reading

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Citations

Mar 20, 2012·Cell and Tissue Research·Clémence BelleannéeRobert Sullivan
Jan 13, 2009·Human Reproduction Update·Gail A Cornwall
Jun 26, 2009·Biology of Reproduction·Elizabeth M SnyderMichael D Griswold
Jan 10, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·P SipiläM Poutanen
Aug 26, 2006·Biochemical and Biophysical Research Communications·Jenni JalkanenMatti Poutanen
Dec 21, 2013·Scandinavian Journal of Urology·Mikael LehtihetAke Pousette
Jun 13, 2015·Journal of Molecular Endocrinology·Fernanda N CavalcantiCatarina S Porto
May 26, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Petra Sipilä, Ida Björkgren
Apr 1, 2018·The FEBS Journal·Atsushi AsanoAlexander J Travis
Aug 16, 2014·Quarterly Reviews of Biophysics·David Sept, Anders E Carlsson
Nov 10, 2018·Basic and Clinical Andrology·Joël R Drevet
Dec 6, 2017·Journal of Anatomy·Jennifer R Hughes, Trish Berger
Oct 12, 2007·Biology of Reproduction·Evemie DubéDaniel G Cyr
Feb 17, 2019·Nature Communications·Fu-Ping ZhangJorma J Palvimo

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Methods Mentioned

BETA
chip

Software Mentioned

Affymetrix
SigmaStat
MAS5
SOM
Bioconductor
Bioconductor R package Affy
- PAK
Bioconductor R package
GeneSpring
R

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