PMID: 8962720Jan 1, 1996Paper

Differential endothelial migration and proliferation to basic fibroblast growth factor and vascular endothelial growth factor

Growth Factors
A YoshidaB R Zetter

Abstract

Neovascularization is a feature of a variety of pathological processes. We compared the characteristics of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) on migration and proliferation of human umbilical vein endothelium (HUVEC). Both VEGF and bFGF induced endothelial cell migration at similar concentrations (1/2 max. VEGF = approximately 1.0 ng/ml, bFGF = approximately 5.0 ng/ml). However, VEGF-stimulated migration was two-fold greater than bFGF at 1 and 10 ng/ml (p < 0.05). In contrast, bFGF induced proliferation four-fold more effectively than VEGF (1/2 max. 1 ng/ml and 1.4 ng/ml respectively). Checkerboard migration assays for bFGF showed a predominantly chemokinetic pattern, whereas VEGF was predominantly chemotactic. VEGF and bFGF were not synergistic in monolayer proliferation and migration assays. Three angiogenesis inhibitors, alpha-interferon, TNP-470, and platelet factor-4, inhibited VEGF and bFGF induced cell migration. These results indicate that VEGF and bFGF are chemoattractants that stimulate endothelial migration by different mechanisms and that both can be inhibited by known angiogenesis inhibitors.

References

May 16, 1990·Journal of the National Cancer Institute·R J SharpeT E Maione
Jun 1, 1990·Biochimica Et Biophysica Acta·C H Blood, B R Zetter
Jan 23, 1987·Science·J Folkman, M Klagsbrun
Jan 1, 1987·Nouvelle Revue Française D'hématologie·A Camez, G Tobelem
Jul 1, 1989·The Journal of Cell Biology·D B Rifkin, D Moscatelli
Jun 15, 1989·Biochemical and Biophysical Research Communications·N Ferrara, W J Henzel
Feb 1, 1982·The Journal of Cell Biology·H SeppäG R Martin
Oct 1, 1993·Journal of Pediatric Surgery·H BremJ Folkman
Mar 1, 1993·Journal of Pediatric Surgery·H Brem, J Folkman

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Citations

Sep 23, 2009·Archives of Otolaryngology--head & Neck Surgery·Weimin LiJizhen Lin
Mar 10, 2001·The Journal of Pathology·C von BülowV Nehls
Dec 31, 2011·Langenbeck's Archives of Surgery·Koji TamagawaTakao Ando
Sep 18, 2008·Angiogenesis·Pasquale CironeCraig M Crews
Apr 28, 2001·Prostaglandins & Other Lipid Mediators·T S PanettiD F Mosher
Jan 5, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Louis M MessinaRajabrata Sarkar
Mar 6, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kuen Yong LeeDavid J Mooney
Feb 25, 2003·Protein Expression and Purification·Zhi ShengWilliam J Chirico
Oct 1, 1996·Cytokine & Growth Factor Reviews·M Klagsbrun, P A D'Amore
Nov 14, 1997·Nature Medicine·M SkobeN E Fusenig
Aug 6, 2000·British Journal of Haematology·M MinischettiA F Dammacco
Oct 22, 2008·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Kevin L TaylorDaniel J Lindner
Mar 3, 2011·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Joris ErdmannCasper H J van Eijck
Apr 20, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Brad A BryanPatricia A D'Amore
Nov 19, 2011·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Tejas DesaiWilliam Kirby
Oct 3, 2007·The Journal of Clinical Investigation·Lars Johan NissenYihai Cao
Jul 5, 2003·Chemical & Pharmaceutical Bulletin·Ashraf Hassan AbadiGhaneya Sayed Hassan
Mar 8, 2005·The Journal of Reproduction and Development·Koji IijimaEimei Sato
Jul 24, 2008·PloS One·Sallouha AidoudiAndreas Bikfalvi
Mar 13, 2014·PloS One·Eileen M Weinheimer-HausTimothy J Koh
Apr 25, 2000·Drugs·H H Sedlacek
Nov 1, 2002·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Sundar SanthanamKen O'Byrne
Apr 4, 2014·Biological & Pharmaceutical Bulletin·Yoshimichi SaiEmi Nakashima
Mar 5, 2014·Biomaterials·Kara L SpillerGordana Vunjak-Novakovic
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·J M RosensteinJ M Krum
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Keisuke WakasugiPaul Schimmel
May 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ying YuDavid S Milstone
Aug 25, 1999·Microvascular Research·C L GrosskreutzP A D'Amore
Dec 31, 1998·In Vitro Cellular & Developmental Biology. Animal·Z ParandooshM P Nova
Jan 5, 2002·Cell Communication & Adhesion·N SogaK A Hruska
Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·J T PriceE C Kohn
Jan 9, 2001·The Journal of Investigative Dermatology. Symposium Proceedings·R Klafter, J L Arbiser
Aug 13, 2011·Biochemical and Biophysical Research Communications·Hideki OkazakiKoji Sayama
Jul 19, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·A ZierisC Werner

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