Differential epithelial and stromal protein profiles in cone and non-cone regions of keratoconus corneas

Scientific Reports
Gary Hin-Fai YamJodhbir S Mehta

Abstract

Keratoconus (KC) is an ectatic corneal disease characterized by progressive thinning and irregular astigmatism, and a leading indication for corneal transplantation. KC-associated changes have been demonstrated for the entire cornea, but the pathological thinning and mechanical weakening is usually localized. We performed quantitative proteomics using Sequential Windowed Acquisition of All Theoretical Fragment Ion Mass Spectrometry (SWATH-MS) to analyze epithelial and stromal changes between the topographically-abnormal cone and topographically-normal non-cone regions of advanced KC corneas, compared to age-matched normal corneas. Expression of 20 epithelial and 14 stromal proteins was significantly altered (≥2 or ≤0.5-fold) between cone and non-cone in all 4 KC samples. Ingenuity pathway analysis illustrated developmental and metabolic disorders for the altered epithelial proteome with mitochondrion as the significant gene ontology (GO) term. The differential stromal proteome was related to cellular assembly, tissue organization and connective tissue disorders with endoplasmic reticulum protein folding as the significant GO term. Validation of selected protein expression was performed on archived KC, non-KC and normal corneal ...Continue Reading

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Citations

Oct 28, 2019·Omics : a Journal of Integrative Biology·Vishal ShindeShukti Chakravarti
Sep 8, 2019·Progress in Retinal and Eye Research·Andri K RiauJodhbir S Mehta
Dec 17, 2020·Asia-Pacific Journal of Ophthalmology·Rohit ShettySwaminathan Sethu
May 23, 2020·Experimental Eye Research·Gary H F YamVishal Jhanji
Mar 27, 2021·Current Ophthalmology Reports·Jingwen CaiYutao Liu

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Methods Mentioned

BETA
protein folding
dissection
electrophoresis
fluorescence microscopy

Software Mentioned

ProteinPilot
DAVID ( Database for Annotation , Visualization and Integrated...
Peakview
Quantity One
IPA
DAVID Bioinformatics Resources
Pentacam
DAVID
Cluster

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