Mar 31, 2020

CD8+ T-cell transcription and DNA methylation show age specific differences and lack correlation with clinical outcome in pediatric Inflammatory Bowel Disease

BioRxiv : the Preprint Server for Biology
Konstantin KozlovMatthias Zilbauer


Background & Aims: CD8+ T-cell gene expression has been implicated in the pathogenesis of Inflammatory Bowel Diseases (IBD) and has been shown to correlate with disease outcome in adult patients. Moreover, CD8+ T-cell exhaustion was identified as a contributing mechanism that impacts on disease behaviour. We aimed to explore CD8+ T-cell gene expression and DNA methylation in children newly diagnosed with IBD and test their correlation with disease outcome. Methods: We prospectively recruited 112 children with IBD at the point of diagnosis and 19 non-IBD controls. Follow-up samples were obtained from a subset of patients at 3-month intervals (n=62). CD8+ T-cells were purified from peripheral blood samples using magnetic bead sorting and genome-wide transcriptional (n=192) and DNA methylation (n=66) profiles were generated using Affymetrix and Illumina arrays respectively. Publicly available adult CD8+ T-cell transcriptomes and DNA methylomes were included in data analyses to investigate age dependent differences. Results: Variation amongst CD8+ T-cell transcriptomes obtained from children showed association with disease, systemic inflammation, age and gender, but lacked correlation with disease outcome in pediatric IBD. In contr...Continue Reading

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Experimental drug
Drug Evaluation
Pharmaceutical Preparations
Genetic Structures
Biological Evolution

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