Differential evolutionary wiring of the tyrosine kinase Btk.

PloS One
Hossain M NawazJessica M Lindvall

Abstract

A central question within biology is how intracellular signaling pathways are maintained throughout evolution. Btk29A is considered to be the fly-homolog of the mammalian Bruton's tyrosine kinase (Btk), which is a non-receptor tyrosine-kinase of the Tec-family. In mammalian cells, there is a single transcript splice-form and the corresponding Btk-protein plays an important role for B-lymphocyte development with alterations within the human BTK gene causing the immunodeficiency disease X-linked agammaglobulinemia in man and a related disorder in mice. In contrast, the Drosophila Btk29A locus encodes two splice-variants, where the type 2-form is the more related to the mammalian Btk gene product displaying more than 80% homology. In Drosophila, Btk29A displays a dynamic pattern of expression through the embryonic to adult stages. Complete loss-of-function of both splice-forms is lethal, whereas selective absence of the type 2-form reduces the adult lifespan of the fly and causes developmental abnormalities in male genitalia. Out of 7004-7979 transcripts expressed in the four sample groups, 5587 (70-79%) were found in all four tissues and strains. Here, we investigated the role of Btk29A type 2 on a transcriptomic level in larval ...Continue Reading

References

Apr 11, 1975·Science·M C King, A C Wilson
Jan 1, 1992·Developmental Genetics·D T Dang, N Perrimon
Sep 29, 1998·Biochemical Pharmacology·F M Uckun
Jul 3, 1999·The Journal of Biological Chemistry·L YaoT Kawakami
Jan 15, 2000·Science·M TatenoT Adachi-Yamada
May 1, 2001·Journal of Cellular Biochemistry·S MukhopadhyayD Dash
May 8, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C I SmithM Vihinen
Aug 3, 2001·Cell Structure and Function·H KuwabaraN Sawada
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Veronica F HinmanEric H Davidson
Jun 24, 2004·European Journal of Immunology·Jessica M LindvallTahmina C Islam
Jun 28, 2005·Developmental Biology·Inna DjagaevaSteven K Beckendorf
Aug 23, 2005·Developmental Biology·David R Angelini, Thomas C Kaufman
Jun 13, 2006·Biochemical and Biophysical Research Communications·Jessica M LindvallC I Edvard Smith
May 31, 2007·Nature Genetics·Venkateswara R ChintapalliJulian A T Dow
Sep 18, 2007·Advances in Immunology·John G Monroe, Kenneth Dorshkind
Jun 17, 2008·Cell·Jeffrey M SkerkerMichael T Laub
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shruti SharmaWenxia Song
Jan 14, 2009·Nature Reviews. Genetics·Douglas H Erwin, Eric H Davidson
Feb 14, 2009·PLoS Genetics·Renee D ReadJohn B Thomas
Oct 12, 2010·American Journal of Physiology. Renal Physiology·Julian A T Dow, Michael F Romero
May 31, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chaohong LiuWenxia Song
Oct 21, 2011·Nature·David BrawandHenrik Kaessmann

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Citations

May 16, 2013·Scandinavian Journal of Immunology·L VargasC I E Smith

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Methods Mentioned

BETA
chips
PCR
in vitro transcription

Software Mentioned

Distal
Bioconductor
DAVID
affy
GSEA
Excel
Affymetrix
BiNGO
R
Cytoscape

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