Differential expression analysis comparing EBV uninfected to infected human cell lines identifies induced non-micro small non-coding RNAs

Non-coding RNA Research
Lumbini I MossWalter N Moss

Abstract

Epstein-Barr virus (EBV) is a ubiquitous human herpes virus, which is implicated in cancer and various autoimmune diseases. This study profiles non-micro small non-coding RNA expression changes induced by latent EBV infection. Using small RNA-Seq, 346 non-micro small RNAs were identified as being significantly differentially expressed between EBV(+) BJAB-B1 and EBV(-) BJAB cell lines. Select small RNA expression changes were experimentally validated in the BJAB-B1 cell line as well as the EBV-infected Raji and Jijoye cell lines. This latter analysis recapitulated the previously identified induction of vault RNA1, while also finding novel evidence for the deregulation of several tRNAs and a snoRNA.

Datasets Mentioned

BETA
GSE139425

Methods Mentioned

BETA
reverse
RNA-Seq

Software Mentioned

HISAT2
R Ballgown
LC Sciences
miR
new
FastQC
Trim Galore
Ballgown
genefilter
StringTie

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