Differential expression and localization of CFTR and ENaC in mouse endometrium during pre-implantation

Cell Biology International
Jian Zhi YangHsiao Chang Chan

Abstract

Interaction between the cystic fibrosis transmembrane conductance regulator (CFTR), a CAMP-activated Cl- channel, and epithelial Na+ channel (ENaC) has been proposed as the major mechanism regulating uterine fluid absorption and secretion. Differential expression of these ion channels may give rise to dynamic changes in the fluid environment affecting various reproductive events in the female reproductive tract. This study investigated the expression and localization of CFTR and ENaC during the pre-implantation period. Semi-quantitative reverse transcriptase polymerase chain reaction and immunohistochemistry were used to study the expression and localization of CFTR and ENaC in uteri collected from mature superovulated female mice. RT-PCR showed maximal ENaC and CFTR expression on day 3 after mating. Maximal immunoreactivity was also observed for both ENaC and CFTR on day 3 after mating. However, ENaC was immunolocalized to the apical membrane of both luminal and glandular epithelia, while CFTR was predominantly found in the stromal cells rather than the epithelial cells. Differential expression and localization of CFTR and ENaC provide a molecular mechanism by which maximal fluid absorption can be achieved immediately prior to...Continue Reading

Citations

Jan 28, 2006·The Journal of Membrane Biology·N SallehS R Milligan
Jan 8, 2013·Molecular Aspects of Medicine·Shuang ZhangD Randall Armant
Jan 27, 2012·American Journal of Physiology. Cell Physiology·Yawar J QadriCatherine M Fuller
Mar 5, 2014·Human Reproduction Update·Ye Chun RuanHsiao Chang Chan
Sep 5, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Xiao SunHsiao Chang Chan
Jun 28, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hai-Yan LinCheng Zhu
Nov 19, 2008·The Journal of Physiology·Hsiao Chang ChanZhen Zhou
May 27, 2010·International Journal of Andrology·N MannowetzG Wennemuth
Jan 18, 2006·Molecular and Cellular Endocrinology·Hsiao Chang ChanYu Ying Yuan
Jan 21, 2012·Biology of Reproduction·Ying LiuLi-Ming Chen
Jul 23, 2014·Molecular Endocrinology·Sandeep PawarMilan K Bagchi
Nov 22, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yiyun LouFan Jin
May 3, 2014·Physiology·Xin-Mei LiuHe-Feng Huang
Feb 23, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Xian XiaMing Yue
Jan 10, 2018·The Journal of Reproduction and Development·Kohta KikuchiMasashi Takahashi
Feb 13, 2020·International Journal of Molecular Sciences·Gaetano RiemmaPasquale De Franciscis
Feb 6, 2021·Scientific Reports·Octavio López-AlborsFrancisco Alberto García-Vázquez
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Florian LangMadhuri S Salker
Jun 17, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yue ZhangYanqing Geng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.