Differential expression and localization of neuronal intermediate filament proteins within newly developing neurites in dissociated cultures of Xenopus laevis embryonic spinal cord

Cell Motility and the Cytoskeleton
J Undamatla, Ben G Szaro

Abstract

The molecular subunit composition of neurofilaments (NFs) progressively changes during axon development. In developing Xenopus laevis spinal cord, peripherin emerges at the earliest stages of neurite outgrowth. NF-M and XNIF (an alpha-internexin-like protein) appear later, as axons continue to elongate, and NF-L is expressed after axons contact muscle. Because NFs are the most abundant component of the vertebrate axonal cytoskeleton, we must understand why these changes occur before we can fully comprehend how the cytoskeleton regulates axon growth and morphology. Knowing where these proteins are localized within developing neurites and how their expression changes with cell contact is essential for this understanding. Thus, we examined by immunofluorescence the expression and localization of these NF subunits within dissociated cultures of newly differentiating spinal cord neurons. In young neurites, peripherin was most abundant in distal neuritic segments, especially near branch points and extending into the central domain of the growth cone. In contrast, XNIF and NF-M were usually either absent from very young neurites or exhibited a proximal to distal gradient of decreasing intensity. In older neurites, XNIF and NF-M expres...Continue Reading

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Citations

Sep 28, 2007·Histochemistry and Cell Biology·Meagan BarclayGary D Housley
Sep 16, 2004·Nature Reviews. Molecular Cell Biology·Lynne Chang, Robert D Goldman
Nov 5, 2003·Molecular Biology of the Cell·Brian T HelfandRobert D Goldman
Jan 19, 2012·Journal of Biomedical Science·Wen-Ching LeeChung-Liang Chien
Oct 30, 2007·Neuroscience·F LallemendB Malgrange
Apr 13, 2007·Experimental Cell Research·Amar ThyagarajanBen G Szaro
May 2, 2003·The Journal of Comparative Neurology·Christine GervasiBen G Szaro
Nov 5, 2003·Journal of Neurobiology·Roxanne C Lariviere, Jean-Pierre Julien
Feb 11, 2011·Developmental Neurobiology·Gianluca Gallo
Nov 10, 2010·The European Journal of Neuroscience·Kurt M GibbsBen G Szaro
Jan 18, 2017·Genesis : the Journal of Genetics and Development·Paula G SlaterLaura Anne Lowery
Apr 5, 2017·Cold Spring Harbor Perspectives in Biology·Aidong YuanRalph A Nixon
Feb 26, 2016·Journal of Cell Science·Chen Wang, Ben G Szaro
Aug 30, 2008·Development·Yuanyuan LiuBen G Szaro

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