PMID: 2507375Jul 1, 1989Paper

Differential expression and regulation of thyrotropin (TSH) in T cell lines

Molecular and Cellular Endocrinology
D V HarbourW J Meyer

Abstract

The immune system's production of and response to thyrotropin (TSH) suggests the existence of a hypothalamic-lymphoid-thyroid regulatory axis (HLT). To evaluate the possible roles of lymphocyte-derived TSH we characterized its structure and regulation of production. Using an in vitro system, we screened T cell lines for the production of TSH in response to thyrotropin-releasing hormone (TRH). We identified MOLT 4 T cells as producers of intact TSH based on TSH radioimmunoassay (RIA) displacement and de novo synthesized protein structure similar to pituitary TSH. Northern blot analysis using a cDNA probe to TSH beta showed the existence of an mRNA species similar in molecular size to pituitary mRNA of TSH beta. The MOLT 4-derived TSH is induced in a dose-dependent fashion by TRH and that induction is significantly inhibited by the thyroid hormone, triiodothyronine (T3). HUT 78 T cells express an mRNA species similar to TSH beta but do not express detectable protein suggesting a lack of translation of the mRNA species. The evidence suggests that the MOLT 4 T cells serve as an in vitro model for TSH production and may be used to study differential expression of the genes for TSH in the immune system. Elucidation of this in vitro s...Continue Reading

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Citations

Oct 1, 1992·Cellular and Molecular Neurobiology·T L KaoW J Meyer
Dec 12, 2001·Molecular and Cellular Endocrinology·A E Murphy, S Harvey
Apr 23, 2008·Thyroid : Official Journal of the American Thyroid Association·Leonidas H Duntas, David S Cooper
Jan 6, 2009·Thyroid : Official Journal of the American Thyroid Association·Su He Wang, Ronald J Koenig
Sep 10, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jeremy S Schaefer, John R Klein
Apr 15, 1992·Annals of the New York Academy of Sciences·T L KaoW J Meyer
Jun 9, 2012·Journal of Veterinary Internal Medicine·M CamposS Daminet
Sep 24, 2015·European Journal of Endocrinology·Eleonore Fröhlich, Richard Wahl
Sep 30, 2014·Acta Microbiologica Et Immunologica Hungarica·György Csaba
Nov 15, 2012·Cytokine·Staley A Brod, Victoria Bauer
Apr 30, 2014·Frontiers in Immunology·John R Klein
Jun 15, 2016·Developmental and Comparative Immunology·B M Lidy Verburg-van KemenadeMagdalena Chadzinska
Mar 21, 2018·The Journal of Endocrinology·Mone ZaidiTony Yuen
Mar 4, 2006·Experimental Biology and Medicine·John R Klein
Jan 1, 1993·The American Journal of the Medical Sciences·M E PeeleK D Burman
Mar 28, 1997·Science·J WangJ R Klein

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