Differential expression and release of cytokines by an in vitro reconstructed human epidermis following exposure to skin irritant and sensitizing chemicals

Toxicology in Vitro : an International Journal Published in Association with BIBRA
A CoquetteY Poumay

Abstract

In the present study, a model of reconstructed human epidermis (RHE) was used as an in vitro model to discriminate the effects of Tween 80, Triton X100 and benzalkonium chloride (BC) as irritants and 1-chloro-2,4-dinitrobenzene (DNCB) as sensitizing agent. The extent of epidermal irritation and sensitization was evaluated morphologically and on the basis of intracellular and extracellular levels of interleukin-1alpha (IL-1alpha) and interleukin-8 (IL-8). The corresponding constitutive mRNA levels were quantified and the cytotoxic response was assessed by MTT assay. The RHE resembled normal human epidermis with all typical layers. The keratin 10 (K10) was typically confined in the suprabasal layers of the tissue, suggesting normal epidermal terminal differentiation. Topical application of the three surfactants resulted in significant changes of tissue morphology and was coupled with different dose-dependent decreases in cell viability corresponding to their in vivo irritant potency. The IL-1alpha release increased concomitantly with cell viability decrease, but surprisingly, the surfactants did not elicit elevated IL-8 levels. In contrast, DNCB did not induce elevated IL-1alpha release although it induced a rapid dose-dependent ...Continue Reading

References

Mar 1, 1992·Contact Dermatitis·J N Barker
Jan 26, 1991·Lancet·J N BarkerB J Nickoloff
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A H Enk, S I Katz
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A M WangD F Mark
Oct 1, 1968·The British Journal of Dermatology·F R Bettley
May 1, 1995·International Archives of Allergy and Immunology·J Knop, A H Enk
Jan 1, 1995·Skin Pharmacology : the Official Journal of the Skin Pharmacology Society·M Ponec, J Kempenaar
Apr 1, 1993·Journal of Pharmaceutical Sciences·M RegnierH Schaefer
Apr 1, 1993·Journal of Clinical Pathology·A W RowbottomJ P Sloane
Jan 1, 1996·Journal of Applied Toxicology : JAT·M R HollidayI Kimber
Jun 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·O DoucetL Zastrow
Oct 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F G BurlesonG F Gerberick
Oct 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L A RheinsT A Donnelly
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E BellE Johnson
Jul 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R GayA Ernesti
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F M DicksonD J Benford

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Citations

Oct 31, 2006·Archives of Dermatological Research·Yves Poumay, Alain Coquette
Oct 22, 2005·Toxicology and Applied Pharmacology·Sari PappinenArto Urtti
Mar 30, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Thomas WelssKlaus R Schröder
Apr 17, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M K RobinsonJ H Fentem
Mar 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C A RyanG F Gerberick
Feb 22, 2001·Free Radical Biology & Medicine·J FuchsM Podda
Feb 3, 2009·Biological & Pharmaceutical Bulletin·Makoto NiwaMasuko Kobori
Mar 1, 2012·Cellular and Molecular Bioengineering·Nripen S SharmaMartin L Yarmush
Apr 3, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sari PappinenArto Urtti
Sep 8, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Carine TornierNathalie Alépée
Sep 8, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Nathalie AlépéeAnne de Brugerolle de Fraissinette
Oct 11, 2003·Photodermatology, Photoimmunology & Photomedicine·Christelle GélisPatricia Vicendo
May 7, 2011·Journal of Biomedical Materials Research. Part a·Paweena UppananSiriporn Tanodekaew
Jun 22, 2010·Biotechnology and Bioengineering·I CantònJ W Haycock
Apr 4, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Juan SunJunichi Misumi
Feb 6, 2014·Journal of Applied Toxicology : JAT·Muriel Guyard-NicodèmeMarc G J Feuilloley
Jul 19, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Yutaka KimuraSetsuya Aiba
Aug 22, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J MolinariC Barrichello
Aug 3, 2016·Toxicology Letters·Sarah LecasSophie Achard
Apr 21, 2010·Expert Review of Clinical Immunology·Iris S Ale, Howard A Maibacht
Oct 1, 2005·Alternatives to Laboratory Animals : ATLA·David BasketterAndrew Worth
Jan 7, 2020·Alternatives to Laboratory Animals : ATLA·Bianca MariglianiAnna Maria Buehler
Aug 21, 2019·Regulatory Toxicology and Pharmacology : RTP·Renato Ivan de ÁvilaMarize Campos Valadares

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