Differential expression of a human kallikrein 5 (KLK5) splice variant in ovarian and prostate cancer

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Lisa KurlenderEleftherios P Diamandis

Abstract

The presence of more than one mRNA form is common among kallikrein genes. We identified an mRNA transcript of the human kallikrein gene 5 (KLK5), denoted KLK5 splice variant 1 (KLK5-SV1). This variant has a different 5'-splice site, but encodes the same protein as the classical KLK5 transcript. RT-PCR analysis of this variant transcript expression in 29 human tissues indicated highest expression in the cervix, salivary gland, kidney, mammary gland, and skin. Comparative analysis of the expression levels of KLK5-SV1, another splice variant named KLK5 splice variant 2 (KLK5-SV2), and the classical KLK5 form showed that out of all three mRNA transcripts, the classical form is predominantly expressed (found in more tissues and at higher expression levels) followed by KLK5-SV1. KLK5-SV1 is expressed at high levels in ovarian, pancreatic, breast and prostate cancer cell lines. KLK5-SV1 was also found to be expressed in 9/10 ovarian cancer tissues, but it was not found in one normal ovarian tissue tested. Hormonal regulation experiments suggest that KLK5-SV1 is regulated by steroid hormones in the BT-474 breast cancer cell line. Furthermore, this variant had significantly higher expression in normal prostate tissues compared to their ...Continue Reading

Citations

May 10, 2005·Biochimica Et Biophysica Acta·Lisa KurlenderEleftherios P Diamandis
Nov 2, 2004·Nature Reviews. Cancer·Carla A Borgoño, Eleftherios P Diamandis
Jan 4, 2005·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·George M YousefEleftherios P Diamandis
Jan 4, 2005·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Phil D Rye, Torgny Stigbrand
May 9, 2014·BioMed Research International·Sung Kyu Hong
Oct 30, 2019·Cancer Metastasis Reviews·Afshin MoradiJyotsna Batra
Jul 17, 2007·Biochimica Et Biophysica Acta·Georgios Pampalakis, Georgia Sotiropoulou

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