Differential expression of a new isoform of DLG2 in renal oncocytoma.

BMC Cancer
Dmitry ZubakovGyula Kovacs

Abstract

Renal oncocytoma, a benign tumour of the kidney, may pose a differential diagnostic problem due to overlapping phenotype with chromophobe renal cell carcinoma or other types of renal cell tumours. Therefore, identification of molecular markers would be of great value for molecular diagnostics of this tumour type. In the current study we applied various techniques, including Affymetrix microarray hybridization and semiquantitative RT-PCR, to identify genes expressed differentially in renal oncocytomas. Subsequently, we used RACE and Northern blot hybridization to characterize the potential candidates for molecular diagnosis. We have identified new isoform of DLG2 gene, which contains 3'-end exons of the known DLG2 gene along with the hypothetical gene FLJ37266. The new isoform is specifically upregulated in renal oncocytoma, whereas the known DLG2 gene is downregulated in this type of kidney tumour. The new isoform of DLG2 is the promising candidate gene for molecular differential diagnostics of renal oncocytoma.

References

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Citations

Sep 5, 2008·Applied Immunohistochemistry & Molecular Morphology : AIMM·Wei HuangThomas Pier
Oct 5, 2010·The FEBS Journal·Encarnación Muñoz-DelgadoCecilio J Vidal
Feb 20, 2007·European Urology·Olaf ReicheltJörg Schubert
Mar 19, 2014·Oncogene·R Halaoui, L McCaffrey
Jul 5, 2019·Clinical and Experimental Pharmacology & Physiology·Jie LiuPeijun Liu
Mar 24, 2016·Cancer Biomarkers : Section a of Disease Markers·Ahmed MahasMichael Markey
Jun 8, 2017·International Journal of Molecular Sciences·Dinesh KumarJayashankar Das
Aug 1, 2020·Cancers·Alberto M ArenasPedro P Medina

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Datasets Mentioned

BETA
CR933674

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

BLAT
MS
Excel
MJ
Opticon Monitor
Ensembl
Affymetrix Microarray Suit

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