Differential expression of a prophage-encoded glycocin and its immunity protein suggests a mutualistic strategy of a phage and its host

Scientific Reports
E L DenhamJ M van Dijl

Abstract

Sublancin 168 is a highly potent and stable antimicrobial peptide secreted by the Gram-positive bacterium Bacillus subtilis. Production of sublancin gives B. subtilis a major competitive growth advantage over a range of other bacteria thriving in the same ecological niches, the soil and plant rhizosphere. B. subtilis protects itself against sublancin by producing the cognate immunity protein SunI. Previous studies have shown that both the sunA gene for sublancin and the sunI immunity gene are encoded by the prophage SPβ. The sunA gene is under control of several transcriptional regulators. Here we describe the mechanisms by which sunA is heterogeneously expressed within a population, while the sunI gene encoding the immunity protein is homogeneously expressed. The key determinants in heterogeneous sunA expression are the transcriptional regulators Spo0A, AbrB and Rok. Interestingly, these regulators have only a minor influence on sunI expression and they have no effect on the homogeneous expression of sunI within a population of growing cells. Altogether, our findings imply that the homogeneous expression of sunI allows even cells that are not producing sublancin to protect themselves at all times from the active sublancin prod...Continue Reading

References

Nov 1, 1980·Canadian Journal of Microbiology·H E HemphillR Z Korman
Jun 8, 2001·Journal of Bacteriology·T Wiegert, W Schumann
Feb 19, 2002·Molecular Microbiology·Tran Thu HoaDavid Dubnau
Mar 2, 2002·The Journal of Biological Chemistry·Ronald DorenbosJan Maarten Van Dijl
Apr 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·K KobayashiN Ogasawara
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shunji NakanoPeter Zuber
Apr 28, 2005·Molecular Microbiology·Torsten Stein
May 27, 2005·Molecular Microbiology·Jan-Willem VeeningOscar P Kuipers
Nov 4, 2005·Journal of Bacteriology·Graciela L LorcaMilton H Saier
May 16, 2007·Molecular Microbiology·Thijs R H M KouwenJean-Yves F Dubois
Aug 28, 2007·Journal of Bacteriology·Mark A StrauchYoann Le Breton
Apr 17, 2008·Molecular Systems Biology·Jan-Willem VeeningOscar P Kuipers
Oct 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Allison V BanseRichard Losick
Dec 3, 2008·Antimicrobial Agents and Chemotherapy·Jean-Yves F DuboisJan Maarten van Dijl
Sep 10, 2009·Antimicrobial Agents and Chemotherapy·Thijs R H M KouwenJan Maarten van Dijl
Aug 11, 2010·FEMS Microbiology Reviews·Hikmate AbriouelAntonio Gálvez
Jan 5, 2011·Nature Chemical Biology·Trent J OmanWilfred A van der Donk
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Aug 19, 2015·Antimicrobial Agents and Chemotherapy·C V Garcia De GonzaloJ M van Dijl
Oct 27, 2015·Metabolic Engineering·Anne GoelzerVincent Fromion
Oct 30, 2016·Current Opinion in Structural Biology·Gillian E Norris, Mark L Patchett
Nov 8, 2017·ACS Chemical Biology·Subhanip BiswasWilfred A van der Donk
Sep 6, 2018·ACS Chemical Biology·Hengqian RenHuimin Zhao

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Citations

Dec 22, 2020·The ISME Journal·Anna DragošÁkos T Kovács
Jun 9, 2021·Archives of Virology·Katharina Kohm, Robert Hertel

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Methods Mentioned

BETA
environmental stresses
PCR
dissection
fluorescence microscopy

Software Mentioned

SigM
FIJI
ImageJ
TLM
Quant

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