PMID: 8586611May 1, 1995Paper

Differential expression of alpha 1 and alpha 2 chains of type VI collagen in the upper, middle, and lower dermal fibroblasts in vitro

Journal of Biochemistry
T IzumiT Nishikawa

Abstract

Levels of type VI collagen mRNAs were determined in cultured skin fibroblasts derived from the upper, middle, and lower layers. The level of alpha 1 (VI) collagen mRNA was the highest in the fibroblasts from the upper layer. In contrast, alpha 2 (VI) collagen mRNA level in the fibroblasts from the lower layer predominated over those in the upper and middle layers. The level of alpha 3 (VI) collagen mRNA was the same in the three dermal layers. When the fibroblasts from the upper layer were passaged successively, the alpha 1 (VI) collagen mRNA level declined and alpha 2 (VI) collagen mRNA level increased to those of the lower dermal fibroblasts. These results indicate that alpha 1 (VI) and alpha 2 (VI) collagen expressions in skin fibroblast cultures are heterogeneous and that successive passages of the upper dermal fibroblasts may result in transition of the phenotype of alpha 1 (VI) and alpha 2 (VI) expression to that of lower dermal fibroblasts. The modulation of the expression of alpha 1 (VI) and alpha 2 (VI) chains presumably reflects the process of cellular aging in vitro and should be a useful biochemical marker in studies of cell aging.

Citations

Apr 21, 2018·Npj Aging and Mechanisms of Disease·Ingo LämmermannJohannes Grillari
Aug 23, 2019·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Yanhai ZuoHui Chen
Jan 3, 2013·Experimental Dermatology·David JansonAbdoelwaheb El Ghalbzouri
May 28, 2021·Mechanisms of Ageing and Development·Holly N Wilkinson, Matthew J Hardman
Sep 24, 1998·Differentiation; Research in Biological Diversity·I NakajimaH Aso

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