DOI: 10.1101/510602Jan 3, 2019Paper

Differential expression of BMP antagonists, gremlin and noggin in hydra: antagonism between Wnt and BMP pathways

BioRxiv : the Preprint Server for Biology
Lakshmi Surekha KrishnapatiSurendra Ghaskadbi

Abstract

Mechanisms regulating BMP and Wnt signaling pathways have been widely studied in many organisms. One of the mechanisms by which these pathways are regulated is by binding of extracellular ligands. In the present study, we report studies with two BMP antagonists, gremlin and noggin from Hydra vulgaris Ind-Pune and demonstrate antagonistic relationship between BMP and Wnt pathways. Gremlin was ubiquitously expressed from the body column to head region except in the basal disc and hypostome. During budding, gremlin was expressed predominantly in the budding region suggesting a possible role in budding; this was confirmed in polyps with different stages of buds. Noggin, on the other hand, was predominantly expressed in the endoderm of hypostome, base of the tentacles, lower body column and at the basal disc in whole polyps. During budding, noggin was expressed at the sites of emergence of tentacles suggesting a role in tentacle formation. This was confirmed in alsterpaullone-treated polyps, which showed noggin expression as distinct spots where ectopic organizers and ectopic tentacles eventually formed. Using RT-PCR, we found that up-regulation of Wnt is accompanied with down-regulation of BMP5-8b demonstrating antagonism between t...Continue Reading

Related Concepts

Down-Regulation
Hormones, Ectopic
Hydra Polyps
Polyps
Up-Regulation (Physiology)
Vertebrates
Bone Morphogenetic Proteins
Noggin protein
Organ
Site

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