Differential expression of calbindin in nigral dopaminergic neurons in two mice strains with differential susceptibility to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine

Journal of Chemical Neuroanatomy
D J VidyadharaPhalguni Anand Alladi

Abstract

Parkinson's disease (PD) affects the A9 dopaminergic (DA) neurons of substantia nigra pars compacta (SNpc) whereas other DA neuronal subtypes are spared. The role of calbindin in this differential vulnerability has been long elicited, and is seen in the MPTP induced mice models of PD. A peculiar feature of mice models is the strain specific differences in the susceptibility to MPTP. Here, calbindin-D28K expression in DA neurons of SNpc of MPTP susceptible C57BL/6 mice and MPTP resistant CD-1 mice was studied as a susceptibility marker of degeneration. Unbiased stereological estimation of immunoperoxidase stained midbrain sections revealed significantly higher number of calbindin immunoreactive cells in SNpc of CD-1 mice compared to that of C57BL/6 strain. Western blotting showed minimal differences in the levels. Calbindin-tyrosine hydroxylase immunofluorescence co-labeling was performed to map the calbindin immunoreactive DA neurons in SNpc and ventral tegmental area (VTA) and to quantify the calbindin expression at cellular level. While the levels were comparable in VTA of both mice strains, the SNpc of CD-1 mice showed significantly higher calbindin expression. Within the SNpc, the medial and dorsal subdivisions showed highe...Continue Reading

References

Aug 1, 1992·Trends in Neurosciences·K G BaimbridgeJ H Rogers
Feb 1, 1988·Archives of Biochemistry and Biophysics·G E KassS Orrenius
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C R GerfenJ J Miller
Nov 17, 1999·Neuropathology and Applied Neurobiology·S Y MaJ O Rinne
Aug 15, 2002·Neuropathology and Applied Neurobiology·C R CabelloB Pakkenberg
Sep 5, 2002·The Journal of Comparative Neurology·Yaping ChuJeffrey H Kordower
Dec 25, 2002·Neurobiology of Aging·Heiko BraakEva Braak
Sep 16, 2003·Neuron·William Dauer, Serge Przedborski
Mar 26, 2005·Brain Research. Molecular Brain Research·Richard Jay Smeyne, Vernice Jackson-Lewis
May 5, 2006·Movement Disorders : Official Journal of the Movement Disorder Society·Uday B MuthaneAndrew J Lees
Apr 3, 2007·Nature Protocols·Vernice Jackson-Lewis, Serge Przedborski
Apr 6, 2007·Trends in Neurosciences·Anders Björklund, Stephen B Dunnett
Nov 17, 2010·Frontiers in Neuroanatomy·Tomás González-HernándezJavier Castro-Hernandez
Jan 19, 2012·The Journal of Comparative Neurology·Stefanie ReyesGlenda M Halliday
Jul 26, 2013·Brain : a Journal of Neurology·Jeffrey H KordowerRaymond T Bartus

❮ Previous
Next ❯

Citations

Jul 10, 2019·Journal of Neurochemistry·D J VidyadharaSreeganga S Chandra
Dec 4, 2019·Frontiers in Aging Neuroscience·Domenico SergiMaria-Grazia Martinoli
Apr 25, 2018·Frontiers in Molecular Neuroscience·S N SureshRavi Manjithaya

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Amygdala and Midbrain Dopamine

The midbrain dopamine system is widely studied for its involvement in emotional and motivational behavior. Some of these neurons receive information from the amygdala and project throughout the cortex. When the circuit and transmission of dopamine is disrupted symptoms may present. Here is the latest research on the amygdala and midbrain dopamine.

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.