Differential expression of calcium-activated chloride channels (CLCA) gene family members in the small intestine of cystic fibrosis mouse models

Histochemistry and Cell Biology
Ina LeverkoehneAchim D Gruber

Abstract

Members of the family of calcium-activated chloride channels (CLCA) have been implicated as modulators of the phenotype in cystic fibrosis (CF). Here, the expression levels of the murine mCLCA1, mCLCA2, mCLCA3 and mCLCA4 were quantified by real-time RT-PCR in the small intestines of CF (cftr (tm1Cam), cftr (TgH(neoim)1Hgu)) and wild type C57BL/6, BALB/c, DBA/2 and NMRI mice. Markedly different expression levels of all four CLCA homologs were observed between the different wild type strains. Expression of mCLCA1 and mCLCA4 was similar in CF versus wild type. In contrast, mCLCA3 mRNA copy numbers were increased up to threefold in all CF models. Immunohistochemical detection of mCLCA3 and PAS reactions on consecutive tissue sections identified a similar increase in mCLCA3 expressing goblet cells, suggesting that elevated mRNA copy numbers of mCLCA3 are due to goblet cell hyperplasia rather than transcriptional regulatory events. Increased mCLCA2 mRNA copy numbers, however, were considered more likely to be due to transcriptional upregulation. Changes in mRNA copy numbers were not associated with altered cell kinetics as determined by immunohistochemistry using antibodies to phospho-histone 3 and activated caspase-3. The results su...Continue Reading

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Citations

Dec 7, 2006·Histochemistry and Cell Biology·Douglas J TaatjesJürgen Roth
Nov 1, 2007·Histochemistry and Cell Biology·Christian ZuberJürgen Roth
Dec 17, 2009·Histochemistry and Cell Biology·Josephine BraunAchim D Gruber
Jun 17, 2011·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Martina WilkeBob J Scholte
May 23, 2008·American Journal of Physiology. Cell Physiology·Chunlei HuanMichael I Kotlikoff
Aug 26, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Layla Al-NakkashJesse Jensen
Feb 13, 2009·BMC Developmental Biology·Marko PiirsooTõnis Timmusk
Jan 10, 2008·Theoretical Biology & Medical Modelling·J Craig Cohen, Janet E Larson
Feb 21, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Melanie K BotheAchim D Gruber
Sep 17, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Stephanie PlogAchim D Gruber
Jul 14, 2010·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Josephine BraunAchim D Gruber
Aug 10, 2006·The Journal of Biological Chemistry·Lars MundhenkAchim D Gruber
Mar 1, 2012·American Journal of Respiratory Cell and Molecular Biology·Lars MundhenkAchim D Gruber
Dec 7, 2007·Gastroenterology·Fiona D YoungRichard F Rozmahel

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