Differential expression of collagen types XVIII/endostatin and XV in normal, keratoconus, and scarred human corneas

Cornea
Marko MäättäTimo Tervo

Abstract

This study was designed to clarify the expression of 2 closely related collagen (Col) types XVIII and XV, and the proteolytically derived endostatin fragment of ColXVIII in normal, keratoconus, and scarred human corneas. Immunohistochemistry, in situ hybridization, immunoelectron microscopy, and Western immunoblotting were used for human corneal samples obtained from penetrating keratoplasty. In the normal cornea, ColXVIII was immunolocalized to the corneal and conjunctival epithelial basement membrane (EBM), Descemet s membrane, and the limbal and conjunctival capillaries. Immunoreaction for endostatin was otherwise similar, but it also was present in corneal epithelial cells. Western immunoblotting showed that normal cornea contains several endostatin fragments ranging from 20 to 100 kDa. ColXV was present in the EBM of the limbus and conjunctiva, but not in EBM of the clear cornea. In situ hybridization revealed that corneal basal epithelial cells were responsible for the synthesis of ColXVIII mRNA. Keratoconus cases were characterized by an irregular EBM immunoreactivity for ColXVIII and endostatin and patchy immunoreactivity beneath EBM. In scarred corneas, highly increased immunoreactivity for ColXVIII, endostatin, and Co...Continue Reading

References

Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y MuragakiB R Olsen
Jan 1, 1995·Progress in Nucleic Acid Research and Molecular Biology·T Pihlajaniemi, M Rehn
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·A Hill, H Ploegh
May 1, 1997·Cornea·M C KenneyA V Ljubimov
Aug 1, 1997·Current Eye Research·A J TuoriH M Uusitalo
Mar 11, 1998·Survey of Ophthalmology·Y S Rabinowitz
Mar 21, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·J SaarelaT Pihlajaniemi
May 18, 1999·The American Journal of Pathology·R ValtolaK Alitalo
Oct 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N MiosgeR Timpl
Mar 16, 2000·The EMBO Journal·U FelborW Mothes
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L J Burke, A Baniahmad
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W BlochS Werner
Apr 20, 2001·The International Journal of Biochemistry & Cell Biology·Y Cao
Jan 10, 2002·Clinical & Experimental Ophthalmology·S A Collier
Feb 6, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Anu MuonaTaina Pihlajaniemi
Apr 3, 2002·The EMBO Journal·Naomi FukaiBjorn R Olsen
May 23, 2002·FEBS Letters·Seo Jin LeeSeung Taek Lee
Jun 22, 2002·Experimental Eye Research·T SherwinG M Clover
Oct 16, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Alberto AuricchioMichael J Tolentino
Dec 31, 2002·Investigative Ophthalmology & Visual Science·Takuji KatoDimitri T Azar
Feb 26, 2003·Investigative Ophthalmology & Visual Science·Hideharu FunatsuSadao Hori
Apr 3, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kyoichi TakahashiPeter A Campochiaro
Apr 23, 2003·American Journal of Medical Genetics. Part a·Susana E KliemannSérgio Rosemberg
Apr 26, 2003·Investigative Ophthalmology & Visual Science·Raghu C MurthyJ Timothy Stout
May 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Charles G Smith, John R Vane
May 27, 2003·Investigative Ophthalmology & Visual Science·Alexander G Marneros, Bjorn R Olsen
Sep 6, 2003·Experimental Eye Research·N H BrookesT Sherwin
Oct 4, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ritva YlikärppäTaina Pihlajaniemi
Nov 15, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Harri ElamaaTaina Pihlajaniemi
Dec 20, 2003·The EMBO Journal·Alexander G MarnerosBjorn R Olsen
Jan 31, 2004·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Shizuya SaikaAkira Ooshima
Nov 5, 2004·The EMBO Journal·Neena S RaneRamanujan S Hegde

❮ Previous
Next ❯

Citations

Apr 14, 2006·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Marko MäättäMarita Uusitalo
Oct 1, 2013·Investigative Ophthalmology & Visual Science·André A M TorricelliSteven E Wilson
Jul 25, 2015·Cell and Tissue Research·Dawiyat MassoudiStephane D Galiacy
Jun 12, 2010·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Miguel Romero-JiménezJames S Wolffsohn
Jan 23, 2013·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Isabella M Y CheungTrevor Sherwin
Feb 18, 2014·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Isabella M Y CheungTrevor Sherwin
Oct 24, 2013·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Isabella My CheungTrevor Sherwin
May 2, 2015·Eye·V GalvisA Acera
May 4, 2015·Journal of Cataract and Refractive Surgery·Bettina SchlatterMartin Zinkernagel
Mar 19, 2014·Acta Ophthalmologica·Agnes KrisztLili Takács
Jul 15, 2018·Human Molecular Genetics·Fatemeh SuriElahe Elahi
Jan 25, 2020·Journal of Cellular and Molecular Medicine·Shipra BhansaliVeena Dhawan
Sep 10, 2020·Frontiers in Cell and Developmental Biology·Sudan PuriVivien J Coulson-Thomas
Feb 6, 2021·Matrix Biology Plus·Sandrine BretaudFlorence Ruggiero
Jun 15, 2007·Current Opinion in Ophthalmology

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.