Differential expression of connexin 43 in mouse mammary cells

Cell Biology International
Teresa LambeFinian Martin

Abstract

In this study we have employed suppressive subtractive hybridization (SSH) analysis to investigate differential gene expression in primary mouse mammary epithelial cells (PMMEC) cultured under mildly apoptotic/quiescent and differentiating conditions. Among a small group of genes whose expression was differentially regulated was connexin 43. In vitro, connexin 43 mRNA and protein were detectable in PMMEC cultured under proliferative or mildly apoptotic conditions. The level of connexin 43 mRNA expression in vivo was also investigated. High levels of expression were found to be associated with the periods of greatest glandular plasticity (pubertal expansion of the mammary tree, early pregnancy and during early involution). Thus, terminally differentiated cells in vivo and in vitro did not express connexin 43 mRNA suggesting that connexin 43 expression, and perhaps facilitated gap junction communication, is associated with undifferentiated progenitor cell populations.

Citations

Jul 31, 2007·The Journal of Membrane Biology·Elizabeth McLachlanDale W Laird
Feb 18, 2016·Cancer Letters·Christina L GrekElizabeth S Yeh
Mar 21, 2012·Archives of Biochemistry and Biophysics·Rekha KarJean X Jiang
Mar 21, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Michael K G StewartDale W Laird
Sep 9, 2018·Journal of Mammary Gland Biology and Neoplasia·Sabreen F FostokRabih S Talhouk

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